Expression of pyruvate kinase M2 in preneoplastic hepatic foci of N-nitrosomorpholine-treated rats
Expression of pyruvate kinase M2 in preneoplastic hepatic foci of N-nitrosomorpholine-treated rats
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DOI：
10.1007/s004280050330
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发表时间：
1999-03-01
期刊：
影响因子：
--
通讯作者：
Eigenbrodt, E
中科院分区：
文献类型：
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作者：
Steinberg, P;Klingelhöffer, A;Eigenbrodt, E
本文分析了饮用水中致癌剂N-亚硝基吗啉（NNM）处理的大鼠肝脏中丙酮酸激酶（PK）同工酶L和M-2的表达。在对照动物中，L-PK表达仅限于肝实质细胞，而M-2-PK在胆管上皮、血管壁、内皮和枯否细胞中检测到。在NNM处理的大鼠中，增殖的卵圆细胞始终为L-PK阴性和M-2-PK阳性，而胆管纤维化的导管细胞明显为L-PK阳性并且共表达M-2-PK。然而，没有观察到导管细胞形态分化为肝细胞样细胞。在储存过多糖原的透明嗜酸性细胞灶中，观察到L-PK强染色。在NNM诱导的糖原缺乏灶中，发生从L-PK到M-2-PK表达的转变。
The expression of the pyruvate kinase (PK) isoenzymes L and M-2 was analysed in the livers of rats treated with the hepatocarcinogenic agent N-nitrosomorpholine (NNM) in the drinking water. In control animals L-PK expression was restricted to liver parenchymal cells, whereas M-2-PK was detected in bile duct epithelial, blood vessel wall, endothelial and Kupffer cells. In rats treated with NNM proliferating oval cells were consistently L-PK negative and M-2-PK positive, while the ductal cells of cholangiofibroses were clearly L-PK positive and coexpressed M-2-PK. However, no morphological differentiation of ductal cells into hepatocyte-like cells was observed. In the clear and acidophilic cell foci storing glycogen in excess strong staining for L-PK was observed. In glycogen-poor foci induced by NNM a shift from L-PK to M-2-PK expression takes place.