Expression of ZEBs in gliomas is associated with invasive properties and histopathological grade
Expression of ZEBs in gliomas is associated with invasive properties and histopathological grade
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DOI：
10.3892/ol.2018.8852
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发表时间：
2018-08-01
期刊：
影响因子：
2.9
通讯作者：
Saitoh, Masao
中科院分区：
文献类型：
--
作者：
Suzuki, Keiko;Kawataki, Tomoyuki;Saitoh, Masao
胶质瘤细胞的侵袭性是与这种肿瘤类型相关的主要临床问题，并且与病理恶性等级相关。ZEB1在神经胶质瘤细胞中高度表达，并与各种类型癌症的侵袭性相关。在本研究中，检测了ZEB 1和ZEB 2的表达，旨在确定ZEB在胶质瘤中的作用。ZEB 1和ZEB 2在本研究中使用的所有胶质瘤细胞中均高度表达。在体外和体内实验中，ZEB1和ZEB2的双重敲除比单独敲除更有效地抑制肿瘤侵袭。ZEB1和ZEB2在II级中表达较少，但在IV级中表达水平较高。重要的是，ZEB阳性细胞在复发性胶质瘤恶性转化中比同一病例的初始II级组织中更丰富。这些结果表明，ZEB 1和ZEB 2的水平与胶质瘤的组织病理学分级和侵袭性呈正相关，表明EF 1家族蛋白（ZEB 1和ZEB 2）可能是有用的预后指标和治疗胶质瘤的目标。
The invasiveness of glioma cells is the predominant clinical problem associated with this tumor type, and is correlated with pathological malignant grade. ZEB1 is highly expressed in glioma cells and associated with the aggressiveness of various types of cancer. In the present study, the expression of ZEB1 and ZEB2 was examined with the aim of determining the role of ZEBs in glioma. ZEB1 and ZEB2 were highly expressed in all glioma cells used in this study. Double knockdown of ZEB1 and ZEB2 suppressed tumor invasiveness more effectively than knockdown of either alone, in both in vitro and in vivo experiments. ZEB1 and ZEB2 were marginally expressed in grade II, but expressed at higher levels in grade IV. Importantly, ZEB-positive cells were more abundant in recurrent glioma with malignant transformation than in initial grade II tissue from the same case. These results indicate that the levels of ZEB1 and ZEB2 are positively correlated with histopathological grade and invasiveness of glioma, suggesting that EF1 family proteins (ZEB1 and ZEB2) could be useful as prognostic markers and therapeutic targets in patients with glioma.