Origin of the actinomycin D insensitive RNA species in Aedes albopictus cells.
Origin of the actinomycin D insensitive RNA species in Aedes albopictus cells.
复制标题
白纹伊蚊细胞中放线菌素 D 不敏感 RNA 种类的起源。
DOI：
10.1093/nar/5.4.1301
复制
发表时间：
1978
影响因子：
14.9
通讯作者：
D. Randlett
中科院分区：
文献类型：
--
作者：
B. Eaton;D. Randlett
在放线菌素 D 或放线菌素 D 与喜树碱或 α-鹅膏菌素的组合存在下。白纹伊蚊细胞合成多种单链RNA。这些放线菌素 D 抗性物种对溴化乙锭敏感，并且它们以 RNase 抗性结构存在于细胞质中，该结构具有线粒体的沉降和浮力密度特征。通过在 7M 尿素中电泳可以检测到 12 种放线菌素 D 不敏感 RNA 种类，其中 11 种与寡 (dT)-纤维素结合。当仅在喜树碱存在的情况下标记白纹伊蚊细胞时，可获得一组相同的寡 (dT)-纤维素结合 RNA 种类。与寡 (dT)-纤维素结合的放线菌素 D 不敏感 RNA 种类与线粒体 DNA 杂交。这些数据表明，放线菌素 D 不敏感的 RNA 种类具有线粒体起源，并且与不明显的污染病毒的复制无关。
In the presence of actinomycin D or a combination of actinomycin D and either camptothecin or alpha-amanatin. Aedes albopictus cells synthesize a variety of single stranded RNA species. These actinomycin D resistant species are ethidium bromide sensitive and they are present in the cell cytoplasm in an RNase resistant structure which has the sedimentation and buoyant density characteristics of mitochondria. Twelve actinomycin D insensitive RNA species can be detected by electrophoresis in 7M urea and 11 of these bind to oligo(dT)-cellulose. An identical set of oligo(dT)-cellulose binding RNA species is obtained when A. albopictus cells are labeled in the presence of camptothecin alone. The actinomycin D insensitive RNA species which bind to oligo(dT)-cellulose hybridize to mitochondrial DNA. These data indicate that the actinomycin D insensitive RNA species have a mitochondrial origin and are not associated with the replication of an inapparent contaminating virus.