Genetic characterization of leaf and stripe rust resistance in the Brazilian wheat cultivar Toropi.
Genetic characterization of leaf and stripe rust resistance in the Brazilian wheat cultivar Toropi.
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DOI：
10.1094/phyto-05-19-0159-r
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发表时间：
2019-09
期刊：
影响因子：
3.2
通讯作者：
S. Rosa;C. M. Zanella;C. Hiebert;A. Brûlé-Babel;H. Randhawa;S. Shorter;L. Boyd;B. McCallum
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文献类型：
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作者：
S. Rosa;C. M. Zanella;C. Hiebert;A. Brûlé-Babel;H. Randhawa;S. Shorter;L. Boyd;B. McCallum
叶锈病和条锈病是全球小麦生产的主要威胁。巴西葡萄品种具有有效的多重防锈性能。 Toropi 使其成为抗锈病基因研究的绝佳选择。使用 2,194 个多态性 90K iSelect SNP 标记对来自不同 Toropi 种子批次的 DNA 进行测试，发现了显着的遗传差异，种子批次之间的相似性高达 35%。因此，我们对衍生自 Toropi 变体 Toropi-6.4 的单一植物系进行了进一步的研究。来自 Toropi-6.4 x Thatcher 杂交的双单倍体群体拥有 168 个品系，多年来在加拿大、新西兰和肯尼亚等地进行了表型分析，共进行了 7 次叶锈病田间试验和 9 次条锈病田间试验。使用 90K iSelect 阵列、SSR 和 KASP 标记进行基因分型，得出 3,043 cM 的遗传图谱，其中包含 1,208 个非冗余标记。在染色体 1B (QLr.crc-1BL/QYr.crc-1BL)、3B (QLr.crc-3BS)、4B (QYr.crc-4BL)、5A（QLr.crc-5AL 和 QYr.crc-5AL）和 5D 上的多个环境中鉴定了源自 Toropi-6.4 的显着数量性状位点 (QTL) （QLr.crc-5DS）。 QTL QLr.crc-1BL/QYr.crc-1BL 与多锈病抗性基因座 Lr46/Yr29 位于同一位置，而 QTL QLr.crc-5DS 位于先前在源自 Toropi 的小麦回交群体中发现的 Lr78 基因座。 QTL组合的比较显示，当与QLr.crc-5AL或QLr.crc-5DS组合时，QLr.crc-1BL显着增强叶锈病抗性，比QLr.crc-5AL和QLr.crc-5DS组合时更显着。当抗条锈病QTL QYr.crc-1BL和QYr.crc-5AL组合时也出现了很强的相加效应。
Leaf and stripe rust are major threats to wheat production worldwide. The effective, multiple rust resistances present in the Brazilian cv. Toropi makes it an excellent choice for a genetic study of rust resistance. Testing of DNA from different seed lots of Toropi with 2,194 polymorphic 90K iSelect SNP markers identified significant genetic divergence, with as much as 35% dissimilarity between seed lots. As a result, further work was conducted with a single plant line derived from Toropi variant Toropi-6.4. A doubled-haploid population with 168 lines derived from the cross Toropi-6.4 x Thatcher was phenotyped over multiple years and locations in Canada, New Zealand and Kenya, with a total of seven field trials undertaken for leaf rust and nine for stripe rust. Genotyping with the 90K iSelect array, SSR and KASP markers resulted in a genetic map of 3,043 cM, containing 1,208 non-redundant markers. Significant quantitative trait loci (QTL) derived from Toropi-6.4 were identified in multiple environments on chromosomes 1B (QLr.crc-1BL/ QYr.crc-1BL), 3B (QLr.crc-3BS), 4B (QYr.crc-4BL), 5A (QLr.crc-5AL and QYr.crc-5AL) and 5D (QLr.crc-5DS). The QTL QLr.crc-1BL/ QYr.crc-1BL co-located with the multi-rust resistance locus Lr46/Yr29, while the QTL QLr.crc-5DS located to the Lr78 locus previously found in a wheat backcross population derived from Toropi. Comparisons of QTL combinations showed QLr.crc-1BL to contribute a significantly enhanced leaf rust resistance when combined with QLr.crc-5AL or QLr.crc-5DS, more so than when QLr.crc-5AL and QLr.crc-5DS were combined. A strong additive effect was also seen when the stripe rust resistance QTL QYr.crc-1BL and QYr.crc-5AL were combined.