Ovarian steroids differentially augment pituitary FSH release in deafferented rats
Ovarian steroids differentially augment pituitary FSH release in deafferented rats
复制标题
卵巢类固醇不同程度地增加入耳聋大鼠的垂体 FSH 释放
DOI：
10.1016/0006-8993(76)90979-3
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发表时间：
1976
期刊：
影响因子：
2.9
通讯作者：
S. Kalra
中科院分区：
文献类型：
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作者：
S. Kalra
大量证据表明，不同的神经解剖部位可能调节垂体LH和FSH的释放4，8，13。Martini及其同事观察到，室旁区（背侧前下丘脑）的电解损伤降低了内侧基底下丘脑（MBH）中可生物测定的FSH释放活性。在戊巴比妥阻断的动情前期大鼠中，Kalra等人8发现，内侧隔区和吻侧内侧视前区（MPOA）的电化学刺激在释放垂体LH方面非常有效，而MPOA-下丘脑前部（AHA）尾部的类似刺激同时诱发FSH和LH的释放。据推测，内侧隔，视前结节和AHA结节途径可能分别参与LH和FSH的释放。从病变2和刺激实验3的研究中已经出现了相当多的支持这一假设的证据，L AHA和MBH之间的解剖学联系的横切阻断了卵巢切除术后的代偿性肥大12和卵巢切除术后外周血中FSH的升高7。在本研究中，我们报告的AHA-MBH神经复合体的POA的手术手段分离的结果在分离的垂体FSH和LH释放诱导的卵巢类固醇治疗。实验在雌性Sprague-Dawley大鼠（Charles River，CD，体重180-220 g）中进行，维持在14 h光照-10 h黑暗循环下。如其他文献所述，用Hala刀（半径和高度为1.8mm）将AHA与POA小心分离。对这些大鼠的脑组织学检查显示，半圆形切口穿过AHA的嘴侧区域并向背侧延伸至室旁核。在假手术对照组大鼠中，将刀降低至颅底但不进行切割。仅将在前下丘脑传入神经阻滞（AHD）后显示持续阴道角化的那些大鼠用于以下两个实验。在实验1（图1）中，AHD大鼠在10.00 h时注射孕酮（P）（4 mg/大鼠，溶于0.1 ml油中）或油（参考文献6）。在P（9只大鼠）或油（5只大鼠）注射前以及15.45和16.45 h，在乙醚麻醉下从每只大鼠的颈静脉抽取血样。用放射免疫法测定血浆中促性腺激素的含量
A considerable body of evidence suggests that different neuroanatomic sites may regulate the pituitary LH and FSH release4, 8, 13. Martini and coworkers observed that electrolytic lesions in the paraventricular region (dorsal anterior hypothalamus) decreased bioassayable FSH releasing activity in the medial basal hypothalamus (MBH). In the pentobarbital-blocked proestrus rats, Kalra et al. 8 found that electrochemical stimulations of the medial septal and the rostral medial preoptic area (MPOA) were highly effective in releasing pituitary LH whereas similar stimulations in the caudal portion of the MPOA-anterior hypothalamus (AHA) evoked simultaneously the release of FSH and LH. It was inferred that a medial septal, preoptico-tuberal and an AHA-tuberal pathway may be involved in the release of LH and FSH, respectively. Considerable supportive evidence for this hypothesis has emerged from studies with lesions 2 and stimulation experiments3, L Transection of the anatomical links between the AHA and the MBH blocked the compensatory hypertrophy following ovariectomy 12 and the post-ovariectomy rise of FSH in the peripheral blood 7. In the present study we report that separation of the AHA-MBH neural complex from the POA by surgical means results in the dissociation of pituitary FSH and LH release induced by treatment with ovarian steroids. The experiments were conducted in female Sprague-Dawley rats (Charles River, CD, body weight 180-220 g) maintained under a 14 h light-10 h dark cycle. The AHA was carefully separated from the POA by a Hala~ z knife (1.8 mm radius and height) as described elsewhereT, 9, lo. Histological examination of the brains of these rats showed that the semicircular cut passed through the rostralmost regions of the AHA and extended dorsally to the paraventricular nucleus. In sham control rats the knife was lowered to the base of the skull but no cut was made, Only those rats which showed persistent vaginal cornification following anterior hypothalamic deafferentation (AHD) were used in two experiments as follows. In experiment 1 (Fig. 1), AHD rats were injected with either progesterone (P)(4 mg/rat in 0.1 ml oil) or oil at 10.00 h (ref. 6). A blood sample from the jugular vein of each rat was drawn under ether anesthesia prior to the P (9 rats) or oil (5 rats) injections and at 15.45 and 16.45 h. Gonadotropins in the plasma obtained from these samples were measured by RIA