Lanosterol and cholesterol have different effects on phospholipid acyl chain ordering.
Lanosterol and cholesterol have different effects on phospholipid acyl chain ordering.
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羊毛甾醇和胆固醇对磷脂酰基链排序有不同的影响。
DOI：
10.1016/0005-2736(85)90470-5
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Yeagle,PL
中科院分区：
文献类型：
--
作者：
Yeagle,PL
在胆固醇和羊毛甾醇的存在下，获得在磷脂的酰基链中的位置9和10处标记的二油酰磷脂酰胆碱的2 H核磁共振（2 H-NMR）谱。在所有情况下，光谱显示三个四极分裂。一个是由于n-1链10位的氘，另一个是由于n-2链10位的氘。第三个氘四极子分裂来自两条链的9位氘。胆固醇，在增加的浓度下，产生从位置9的四极分裂的增加，对应于在膜中包含胆固醇所产生的C-D键段的顺序的增加。注意到对任一链的位置10所产生的四极分裂的影响很小。羊毛甾醇似乎对位置9的四极分裂没有影响。同样，羊毛甾醇对两条链的位置10的四极分裂没有影响。因此，这些数据表明，由于14-甲基，膜结构几乎没有解体。然而，14-甲基阻止羊毛甾醇引起胆固醇特征性磷脂烃链的运动顺序的增加。
2H nuclear magnetic resonance (2H-NMR) spectra of dioleoylphosphatidylcholine labelled at positions 9 and 10 in the acyl chains of the phospholipid were obtained in the presence of cholesterol and lanosterol. The spectra show in all cases three quadrupole splittings. One is due to the deuterium on position 10 of thesn-1 chain and another to the deuterium on position 10 of thesn-2 chain. The third deuterium quadrupole splitting arises from the deuterium at position 9 of both chains. Cholesterol, at increasing concentration, produces an increase in the quadrupole splitting from position 9, corresponding to an increase in order of that C-D bond segment arising from the inclusion of cholesterol in the membrane. Little effect is noted on the quadrupole splittings arising from position 10 of either chain. Lanosterol appears to have no effect on the quadrupole splittings from position 9. Lanosterol, likewise, has no effects on the quadrupole splittings from position 10 of both chains. These data therefore suggest little disorganization of the membrane structure due to the 14-methyl group. However, the 14-methyl group prevents lanosterol from causing the increase in motional order of the phospholipid hydrocarbon chains characteristic of cholesterol.