ALIGNMENT AND DEFECT STRUCTURES IN ORIENTED PHOSPHATIDYLCHOLINE MULTILAYERS
ALIGNMENT AND DEFECT STRUCTURES IN ORIENTED PHOSPHATIDYLCHOLINE MULTILAYERS
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DOI：
10.1016/s0006-3495(79)85225-x
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发表时间：
1979-01-01
影响因子：
3.4
通讯作者：
PERSHAN, PS
中科院分区：
文献类型：
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作者：
ASHER, SA;PERSHAN, PS
二月桂基，二肉豆蔻酰和二棕榈酰磷脂酰胆碱在不同的水浓度到大的定向单域多层膜在高温下退火的对齐是伴随着各种缺陷结构的形成和随后的溶解。这些缺陷中的一些看起来类似于在热致液晶和其他溶致液晶中观察到的那些，反映了这些材料的层状结构。利用偏光显微镜研究了在脂质排列成无缺陷、单畴、多层几何结构过程中缺陷的形成和演变。偏振和暗场显微镜的组合促进表征的缺陷，提出了具体的结构模型。一种新的对齐技术，涉及压缩和膨胀的脂质，其影响样品对齐的温度低于所需的功率技术，进行了描述。较低的温度对齐避免了热分解，如果脂质长时间保持在高温下，有时会发生热分解。利用该技术，在室温下排列具有20重量%水的二月桂酰磷脂酰胆碱的样品（80 μ m厚）。
The alignment of dilauryl-, dimyristoyl- and dipalmitoylphosphatidylcholine at various water concentrations into large oriented monodomain multilayers by annealing at elevated temperatures is accompanied by the formation and subsequent dissolution of various defect structures. Some of these defects appear similar to those observed in thermotropic and other lyotropic liquid crystals, reflecting the lamellar structure of these materials. The formation and evolution of defects during the alignment of the lipids into the defect-free, monodomain, multilamellar geometry was studied using polarized microscopy. A combination of polarized and dark-field microscopy facilitated characterization of the defects; specific structural models are proposed. A new alignment technique involving compression and dilation of the lipid, which effects sample alignment at temperatures that are lower than those required by the Powers technique, is described. Lower temperature alignment avoids thermal decomposition that will sometimes occur if the lipid is maintained at elevated temperatures for prolonged periods. With this technique, samples (80 .mu.m thick) of dilaurylphosphatidylcholine with 20% water by wt were aligned at room temperature.