pH Responsive Polymer Cushions for Probing Membrane Environment Interactions
pH Responsive Polymer Cushions for Probing Membrane Environment Interactions
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DOI：
10.1021/nl200832c
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发表时间：
2011-05-01
期刊：
影响因子：
10.8
通讯作者：
Kuhl, Tonya L.
中科院分区：
文献类型：
--
作者：
El-Khouri, Rita J.;Bricarello, Daniel A.;Kuhl, Tonya L.
报道了一种在动态响应聚合物垫上制备脂质膜的稳健且简单的方法。结构表征表明，可以在聚合物垫上构建完整、填充良好且具有可调节流动性的膜。利用该系统，可以对细胞骨架相互作用引起的膜构象变化进行建模。该膜可以定制，以屏蔽缓冲垫免受 pH 变化的影响，或者通过掺入蛋白质离子通道来快速响应 pH 环境。该基本系统提供了一种复制细胞骨架发生的构象变化的方法，并且对于与底层固体支持物分离的膜特性和膜蛋白相互作用的基础生物物理学研究具有巨大的潜力。
A robust and straightforward method for the preparation of lipid membranes upon dynamically responsive polymer cushions is reported. Structural characterization demonstrates that complete, well-packed membranes with tunable mobility can be constructed on the polymeric cushion. With this system, membrane conformational changes induced by cellular cytoskeleton interactions can be modeled. The membrane can be tailored to screen the cushion from changes in pH or allow rapid response to the pH environment by incorporation of protein ion channels. This elementary system offers a means to replicate the conformational changes that occur with the cellular cytoskeleton and has great potential for fundamental biophysical studies of membrane properties and membrane-protein interactions decoupled from the underlying solid support.