Distribution of alpha-fetoprotein and immunoreactive carcinoembryonic antigen in human hepatocellular carcinoma and hepatoblastoma.
Distribution of alpha-fetoprotein and immunoreactive carcinoembryonic antigen in human hepatocellular carcinoma and hepatoblastoma.
复制标题
甲胎蛋白和免疫反应性癌胚抗原在人肝细胞癌和肝母细胞瘤中的分布。
DOI：
10.1093/oxfordjournals.jjco.a038863
复制
发表时间：
1983
影响因子：
2.4
通讯作者：
T. Mukojima
中科院分区：
文献类型：
--
作者：
S. Hirohashi;Y. Shimosato;Y. Ino;K. Kishi;H. Ohkura;T. Mukojima
应用免疫组织化学方法对62例肝细胞癌（HCC）和5例肝母细胞瘤（HBL）的甲胎蛋白（AFP）和癌胚抗原（CEA）的分布进行了研究，并与患者血清中的抗原水平进行了比较。AFP以粗颗粒状存在于未成熟肿瘤细胞的胞浆中。血清AFP> 400 ng/ml者34例（79.1%）肿瘤细胞呈AFP阳性，1例（4.2%）肿瘤细胞呈AFP阳性。它们存在于8/32例（25%）相对高分化的HCC（Edmondson I或II级），22/32例（68.8%）相对低分化的HCC（Edmondson III或IV级）和5/5例HBL。与常规兔抗CEA免疫球蛋白（DAKO）但不与鼠单克隆抗CEA抗体（Hybritech）免疫反应的抗原存在于非肿瘤性和肿瘤性肝细胞的胆小管表面上。这种CEA交叉反应分化抗原更常见于相对分化良好的肿瘤中，并且肿瘤中该抗原的存在与患者的血清CEA水平无关。总之，癌胚抗原的性质是不产生的HCC或HBL。
The distribution of alpha-fetoprotein (AFP) and immunoreactive carcinoembryonic antigen (CEA) in 62 hepatocellular carcinomas (HCC) and five hepatoblastomas (HBL) all surgically removed was studied by an immunohistochemical method, and the results were compared with the levels of the antigens in the patients' serum. AFP was present as coarse granules in the cytoplasm of immature tumor cells. AFP-positive tumor cells were detected in 34/43 cases (79.1%) with serum AFP levels higher than 400 ng/ml and in 1/24 (4.2%) of the remaining cases. They were present in 8/32 (25%) cases of relatively well-differentiated HCC (Edmondson's grade I or II), 22/32 (68.8%) of relatively poorly differentiated HCC (Edmondson's grade III or IV), and 5/5 of HBL. An antigen immunoreactive with a conventional rabbit anti-CEA immunoglobulin (DAKO) but not with a murine monoclonal anti-CEA antibody (Hybritech) was present on the surface of bile canaliculi of both non-neoplastic and neoplastic hepatocytes. This CEA cross-reactive differentiation antigen was more often found in relatively well-differentiated tumors, and the presence of this antigen in tumors did not correlate with patients' serum CEA levels. In conclusion, CEA of an oncofetal nature was not produced by either HCC or HBL.