P53 EXPRESSION AND DNA-PLOIDY OF CARTILAGE LESIONS-猫眼课题宝

DOI：

10.1016/0046-8177(95)90166-3

发表时间：

1995-06-01

期刊：

HUMAN PATHOLOGY

影响因子：

3.3

通讯作者：

中科院分区：

医学3区

文献类型：

--

作者：

COUGHLAN, B;FELIZ, A;DORFMAN, HD

本研究旨在探讨抑癌基因p53在骨软骨病变中的表达及其与组织学分级和DNA倍体的关系。对p53和Feulgen染色的DNA制备物的免疫组织化学测定进行计算机图像分析。内生软骨瘤、瓶状软骨瘤病和低度（I级和II级）软骨肉瘤为二倍体。高级别（III级）软骨肉瘤和去分化软骨肉瘤的高级别肉瘤成分为非整倍体。去分化软骨肉瘤中分化良好的软骨成分为二倍体。显微镜检查显示，10个内生软骨瘤中有1个为p53弱阳性，6个滑膜软骨瘤病中有1个为p53弱阳性，4个低级别（I级和II级）软骨肉瘤中有3个为p53弱阳性。所有三个高级别（III级）软骨肉瘤p53强阳性。所有4例去分化软骨肉瘤的高级别肉瘤成分均为p53强阳性，而仅在分化良好的软骨区发现局灶性弱阳性。这些结果得到了计算机辅助定量图像分析的证实，结果显示，高级别非整倍体软骨肿瘤的p53表达水平明显高于二倍体低级别恶性肿瘤或良性软骨病变。版权所有（C）1995由W.B.桑德斯公司

The aim of this study was to investigate the expression of a tumor suppressor gene (p53) in cartilage lesions of bone and its relationship to their histological grade and DNA ploidy. An immunohistochemical assay for p53 and Feulgen-stained DNA preparations was subjected to computerized image analysis. Enchondromas, vial chondromatosis, and low grade (grade I and II) chondrosarcomas were diploid. High grade (grade III) chondrosarcomas and high grade sarcomatous components of dedifferentiated chondrosarcomas were aneuploid. Well differentiated cartilaginous components of dedifferentiated chondrosarcomas were diploid. Microscopic examination showed weak focal positivity for p53 in one of 10 enchondromas one of six examples of synovial chondromatosis, and three of four low grade (grade I and II) chondrosarcomas. All three high grade (grade III) chondrosarcomas were strongly positive for p53. The high grade sarcomatous component of all four dedifferentiated chondrosarcomas was strongly positive for p53, whereas only focal weak positivity was noted in the well differentiated cartilaginous areas. These results were confirmed by quantitative computer-assisted image analysis, which showed that high grade aneuploid cartilage tumors demonstrated strikingly higher levels of p53 than did diploid low grade malignant tumors or benign cartilage lesions. Copyright (C) 1995 by W.B. Saunders Company