Ultrastructural evidence of exosome secretion by progenitor cells in adult mouse myocardium and adult human cardiospheres.
Ultrastructural evidence of exosome secretion by progenitor cells in adult mouse myocardium and adult human cardiospheres.
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DOI：
10.1155/2012/354605
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发表时间：
2012
影响因子：
--
通讯作者：
Vassalli G
中科院分区：
文献类型：
--
作者：
Barile L;Gherghiceanu M;Popescu LM;Moccetti T;Vassalli G
尽管只有很少的移植细胞在体内存活，但细胞疗法的有益效果的证明表明，分泌因子可能是这种治疗的活性成分。这一所谓的旁分泌假说得到了以下观察的支持：由祖细胞调节的培养液中含有介导促血管生成和细胞保护作用的生长因子。在半悬浮培养中的心脏前体细胞形成球形簇(心球)，向邻近细胞传递旁分泌信号。旁分泌的一个关键成分是外体，外体是储存在细胞内的膜泡，当这些结构与细胞质膜融合时，就会分泌出来。Exosome是小鼠骨髓CD34+干细胞促血管生成作用的活性成分，也是胚胎间充质干细胞在猪和小鼠心肌梗死后再生的活性成分。在这里，我们提供了小鼠心肌和人心球中祖细胞分泌外切体的电子显微镜证据。外切体是由祖细胞传递旁分泌信号的一个有吸引力的载体。它们可以作为“现成”产品储存。因此，外切体有可能在再生医学的治疗应用中绕过活细胞的许多限制。
The demonstration of beneficial effects of cell therapy despite the persistence of only few transplanted cells in vivo suggests secreted factors may be the active component of this treatment. This so-called paracrine hypothesis is supported by observations that culture media conditioned by progenitor cells contain growth factors that mediate proangiogenic and cytoprotective effects. Cardiac progenitor cells in semi-suspension culture form spherical clusters (cardiospheres) that deliver paracrine signals to neighboring cells. A key component of paracrine secretion is exosomes, membrane vesicles that are stored intracellularly in endosomal compartments and are secreted when these structures fuse with the cell plasma membrane. Exosomes have been identified as the active component of proangiogenic effects of bone marrow CD34+ stem cells in mice and the regenerative effects of embryonic mesenchymal stem cells in infarcted hearts in pigs and mice. Here, we provide electron microscopic evidence of exosome secretion by progenitor cells in mouse myocardium and human cardiospheres. Exosomes are emerging as an attractive vector of paracrine signals delivered by progenitor cells. They can be stored as an “off-the-shelf” product. As such, exosomes have the potential for circumventing many of the limitations of viable cells for therapeutic applications in regenerative medicine.
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DOI：
10.1038/mt.2008.1
发表时间：
2008-04
期刊：
Molecular therapy : the journal of the American Society of Gene Therapy
影响因子：
--
作者：
Dai S;Wei D;Wu Z;Zhou X;Wei X;Huang H;Li G
通讯作者：
Li G
影响因子：
3.7
作者：
Davis DR;Zhang Y;Smith RR;Cheng K;Terrovitis J;Malliaras K;Li TS;White A;Makkar R;Marbán E
通讯作者：
Marbán E
影响因子：
168.9
作者：
Janssens, S;Dubois, C;Van de Werf, F
通讯作者：
Van de Werf, F
影响因子：
5.2
作者：
Andersen, Ditte Caroline;Andersen, Peter;Sheikh, Soren Paludan
通讯作者：
Sheikh, Soren Paludan
影响因子：
5.2
作者：
Ebelt, Henning;Jungblut, Mirco;Braun, Thomas
通讯作者：
Braun, Thomas