Imaging of glucose metabolism by 13C-MRI distinguishes pancreatic cancer subtypes in mice
Imaging of glucose metabolism by 13C-MRI distinguishes pancreatic cancer subtypes in mice
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DOI：
10.7554/elife.46312
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发表时间：
2019-08-13
期刊：
影响因子：
7.7
通讯作者：
Krishna, Murali C.
中科院分区：
文献类型：
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作者：
Kishimoto, Shun;Brendert, Jeffrey R.;Krishna, Murali C.
肿瘤之间和肿瘤内的代谢差异可能是癌症治疗结果的重要决定因素。然而，用于在体内非侵入性地确定这些差异的方法缺乏。以胰腺导管腺癌为模型，我们证明了具有相似遗传背景的肿瘤异种移植物可以通过其不同的13 C标记葡萄糖示踪剂代谢率来区分，这些示踪剂可以通过使用新开发的噪声抑制技术在没有超极化的情况下成像。使用这种方法，基于稳态代谢测量，似乎具有相似代谢谱的癌症亚型可以彼此区分。来自13 C-葡萄糖成像的代谢图将乳酸产生和总体葡萄糖代谢定位于一些肿瘤的不同区域。这种肿瘤异质性在FDG-PET中不会检测不到。
Metabolic differences among and within tumors can be an important determinant in cancer treatment outcome. However, methods for determining these differences non-invasively in vivo is lacking. Using pancreatic ductal adenocarcinoma as a model, we demonstrate that tumor xenografts with a similar genetic background can be distinguished by their differing rates of the metabolism of 13C labeled glucose tracers, which can be imaged without hyperpolarization by using newly developed techniques for noise suppression. Using this method, cancer subtypes that appeared to have similar metabolic profiles based on steady state metabolic measurement can be distinguished from each other. The metabolic maps from 13C-glucose imaging localized lactate production and overall glucose metabolism to different regions of some tumors. Such tumor heterogeneity would not be not detectable in FDG-PET.