Blastocystis subtyping and its association with intestinal parasites in children from different geographical regions of Colombia
Blastocystis subtyping and its association with intestinal parasites in children from different geographical regions of Colombia
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DOI：
10.1371/journal.pone.0172586
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发表时间：
2017-02-21
期刊：
影响因子：
3.7
通讯作者：
Cesar Giraldo, Julio
中科院分区：
文献类型：
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作者：
David Ramirez, Juan;Florez, Carolina;Cesar Giraldo, Julio
芽囊原虫是一种常见的肠道原生生物，大约有10亿多人与各种非人类宿主一起定植。已观察到显著的遗传多样性，导致该属细分为多个亚型(ST)，其中一些亚型仅在非人类宿主中发现。本研究的目的是确定哥伦比亚9个地区有症状(腹痛、肛门瘙痒、腹泻、头痛、恶心和/或呕吐)和无症状儿童中芽囊原虫STS/18S等位基因的分布。作为一项全国性调查的一部分，共收集了2026份粪便样本，以估计儿童肠道寄生虫的频率。最终选择了256份芽囊原虫阳性的样本。样品经DNA提取、实时荧光定量聚合酶链式反应和针对小亚基rRNA基因的芽囊原虫特异性引物测序，用于ST鉴定。在芽囊阳性标本中检出蛔虫(16.4%)、鞭虫(8.2%)、钩虫(7.3%)、十二指肠贾第鞭毛虫(23.1%)、内阿米巴复合体(82%)、结肠内阿米巴(55%)、纳氏膜虫(0.8%)、内囊尾线虫(33.2%)和新巴氏杆菌(2.7%)。共检出ST1(21.4%)、ST2(19.5%)、ST3(55.5%)、ST4(0.8%)、ST6(2%)、ST7(0.8%)等位基因；ST1的等位基因1、2、4、81、82和83；ST2的等位基因9、11、12、15、67、71和73；ST3的等位基因34、36、38、45、49、55、134和128；ST4的等位基因42；ST6的等位基因122；ST7的等位基因142。实施高分辨率分子标记的进一步研究对于了解芽囊原虫的传播动力学以及这种斯特门诺拉在健康和疾病中的作用是必要的。
Blastocystis is a common enteric protist colonizing probably more than 1 billion people with a large variety of non-human hosts. Remarkable genetic diversity has been observed, leading to the subdivision of the genus into multiple subtypes (ST), some of which are exclusively found in non-human hosts. The aim of this study was to determine the distribution of Blastocystis STs/18S alleles in symptomatic (abdominal pain, anal pruritus, diarrhea, headache, nauseas and/or vomit) and asymptomatic children from nine geographical regions of Colombia. A total of 2026 fecal samples were collected as part of a national survey to estimate the frequency of intestinal parasites in children. A set of 256 samples that were Blastocystis positive was finally selected. The samples were submitted to DNA extraction, Real Time PCR and sequencing using Blastocystis-specific primers targeting the small subunit rRNA gene for ST identification. DNA of Ascaris lumbricoides (16.4%), Trichuris trichiura (8.2%), hookworms (Necator americanus/Ancylostoma duodenale) (7.3%), Giardia duodenalis (23.1%), Entamoeba complex (82%), Entamoeba coli (55%), Hymenolepis nana (0.8%), Endolimax nana (33.2%) and Neobalantidium coli (2.7%) was detected in the Blastocystispositive samples. We detected ST1 (21.4%), ST2 (19.5%), ST3 (55.5%), ST4 (0.8%), ST6 (2%) and ST7 (0.8%); alleles 1, 2, 4, 81, 82 and 83 for ST1; alleles 9, 11, 12, 15, 67, 71 and 73 for ST2; alleles 34, 36, 38, 45, 49, 55, 134 and 128 for ST3; allele 42 for ST4; allele 122 for ST6, and allele 142 for ST7. Further studies implementing high-resolution molecular markers are necessary to understand the dynamics of Blastocystis transmission and the role of this Stramenopila in health and disease.