Materials composed of the Drosophila melanogaster protein ultrabithorax are cytocompatible: Ubx Protein Materials are Cytocompatible
Materials composed of the Drosophila melanogaster protein ultrabithorax are cytocompatible: Ubx Protein Materials are Cytocompatible
复制标题
由果蝇蛋白 ultrabithorax 组成的材料具有细胞相容性：Ubx 蛋白材料具有细胞相容性
DOI：
10.1002/jbm.a.34675
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发表时间：
2014
影响因子：
4.9
通讯作者：
Bondos, Sarah E.
中科院分区：
文献类型：
--
作者：
Patterson, Jan L.;Abbey, Colette A.;Bayless, Kayla J.;Bondos, Sarah E.
果蝇Hox蛋白ultrabithorax（Ubx）具有有趣的能力，能够在体外分层自我复制成具有与天然弹性蛋白相当的机械性能的材料。Ubx材料可以很容易地通过基因融合功能化，产生潜在的有用的细胞和组织工程支架。在这里，我们测试了由Ubx或mCherry‐Ubx融合蛋白组成的纤维的细胞相容性。纤维与来源于低传代血管系统的三种原代人细胞系培养：脐静脉内皮细胞、脑血管周细胞或主动脉平滑肌细胞。在任何细胞系中均未观察到对由普通Ubx或mCherry‐Ubx组成的纤维的直接或间接毒性反应。细胞易于粘附于Ubx纤维，并且粘附于纤维的细胞可以在组织培养物之间转移而不丧失活力至少96 h。当连接到纤维，三个细胞系的形态有所不同，但所有的细胞在接触UBX纤维表现出微管网络对齐的UBX纤维的长轴。因此，Ubx纤维与培养的原代人血管细胞是细胞相容的。© 2013 Wiley Periodicals，Inc. J Biomed Mater Res Part A：102A：97-104，2014.
TheDrosophila melanogasterHox protein ultrabithorax (Ubx) has the interesting ability to hierarchically self‐assemblein vitrointo materials that have mechanical properties comparable to natural elastin. Ubx materials can be easily functionalized by gene fusion, generating potentially useful scaffolds for cell and tissue engineering. Here, we tested the cytocompatibility of fibers composed of Ubx or an mCherry‐Ubx fusion protein. Fibers were cultured with three primary human cell lines derived from vasculature at low passage: umbilical vein endothelial cells, brain vascular pericytes, or aortic smooth muscle cells. No direct or indirect toxicity was observed for any cell line, in response to fibers composed of either plain Ubx or mCherry‐Ubx. Cells readily adhered to Ubx fibers, and cells attached to fibers could be transferred between tissue cultures without loss of viability for at least 96 h. When attached to fibers, the morphology of the three cell lines differed somewhat, but all cells in contact with Ubx fibers exhibited a microtubular network aligned with the long axis of Ubx fibers. Thus, Ubx fibers are cytocompatible with cultured primary human vascular cells. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 97–104, 2014.