Changes in E- and N-cadherin expression in developing rat adenohypophysis
Changes in E- and N-cadherin expression in developing rat adenohypophysis
复制标题
DOI：
10.1002/ar.20516
复制
发表时间：
2007-05-01
影响因子：
2
通讯作者：
Yashiro, Takashi
中科院分区：
文献类型：
--
作者：
Kikuchi, Motoshi;Yatabe, Miegumi;Yashiro, Takashi
最近，我们发现，在成年大鼠垂体前叶中，卵泡产生细胞表达N-钙粘蛋白，而滤泡星状细胞和边缘层细胞表达E-钙粘蛋白。这些细胞被认为起源于腺垂体基板的单个细胞群。在本研究中，我们用免疫化学方法检测了垂体组织发生过程中钙粘蛋白类型的差异。从胚胎第11天（E11）至出生后第60天（P60）的大鼠中切除垂体，制备石蜡切片。使用单克隆和多克隆一抗和荧光二抗依次对E-和N-钙粘蛋白进行免疫染色。在E11，E-钙粘蛋白的表达超过口腔上皮，而N-钙粘蛋白的表达仅限于Rathke的囊的原基。当Rathke囊在E13形成时，E-和N-钙粘蛋白在整个细胞群中广泛表达。N-钙粘蛋白在面对管腔的细胞层中表达特别强烈。从E14到E16，大多数细胞表达两种类型的钙粘蛋白;然而，细胞群成为结节表达N-钙粘蛋白，但不表达E-钙粘蛋白。从E18到E20，当出现许多产钙细胞时，仅表达N-钙粘蛋白的细胞数量增加。然而，在远侧部和多层边缘细胞中的一些细胞群体仍然表达两种钙粘蛋白。出生后，大多数细胞只表达一种钙粘蛋白。这些结果可能表明，未确定的腺垂体细胞表达E-和N-钙粘蛋白，但在细胞发生过程中表达E-或N-钙粘蛋白。
Recently, we showed that hormone-producing cells express N-cadherin, while folliculo-stellate cells and marginal layer cells express E-cadherin in the adult rat anterior pituitary gland. These cells are believed to originate from a single cell population of the adenohypophyseal placode. In the present study, we immunohistochemically examined the divergence of cadherin types during pituitary histogenesis. Pituitary glands were excised from rats of embryonic day 11 (E11) through postnatal day 60 (P60) and paraffin sections were prepared. E- and N-cadherins were immunostained sequentially using monoclonal and polyclonal primary antibodies and fluorescent secondary antibodies. At E11, E-cadherin was expressed over oral epithelium, while N-cadherin expression was limited to the primordium of Rathke's pouch. When Rathke's pouch was formed at E13, E- and N-cadherin were broadly expressed in the entire cell population. N-cadherin was expressed particularly intensely in the layer of cells that faced the lumen. From E14 through E16, the majority of cells expressed both types of cadherins; however, the cell population to become the pars tuberalis expressed N-cadherin but not E-cadherin. From E18 through E20, when many hormone-producing cells appear, the number of cells that expressed N-cadherin alone increased. However, some cell populations in the pars distalis and multilayered marginal cells still expressed both cadherins. After birth, most of the cells came to express only one of the cadherin types. These results may suggest that undetermined adenohypophyseal cells express both E- and N-cadherin, but come to express either E- or N-cadherin during cytogenesis.