Human endothelial cell growth and phenotypic expression on three dimensional Poly(Lactide-co-Glycolide) sintered microsphere scaffolds for bone tissue engineering
Human endothelial cell growth and phenotypic expression on three dimensional Poly(Lactide-co-Glycolide) sintered microsphere scaffolds for bone tissue engineering
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DOI：
10.1002/bit.21495
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发表时间：
2007-12-01
影响因子：
3.8
通讯作者：
Laurencin, Cato T.
中科院分区：
文献类型：
--
作者：
Jabbarzadeh, Ehsan;Jiang, Tao;Laurencin, Cato T.
骨组织工程为修复和恢复组织提供了很有前途的替代物。我们实验室利用聚丙交酯-乙交酯共聚物微球开发了一种具有仿生孔结构的三维(3D)多孔生物可吸收支架。骨性愈合和与周围组织的融合部分依赖于多孔结构内新血管的形成。由于内皮细胞在血管生成(从已有的血管系统中形成新的血管)中起着关键作用，本研究的目的是更好地了解人内皮细胞在烧结型Plaga微球支架上的附着、活性、生长和表型表达。扫描电子显微镜(SEM)显示细胞附着在微球表面，并在微球之间架起了孔桥。细胞增殖实验显示细胞数量在培养早期增加，在10~14天达到高峰。免疫荧光染色显示细胞在支架中呈立体增殖，而PECAM-1(血小板内皮细胞黏附分子)染色显示细胞-细胞接触处有典型的定位。基因表达分析显示，生长在Plaga支架上的内皮细胞保持其正常的特征表型。细胞增殖和表型表达与支架孔结构无关。这些结果表明，Plaga烧结型微球支架可以支持人内皮细胞的生长和生物学功能。这项研究的洞察力应该会对未来有所帮助。学习。目的是促进三维组织工程支架的血管生成。
Bone tissue engineering offers promising alter-natives to repair and restore tissues. Our laboratory, has employed poly(lactid-co-glycolide) PLAGA microspheres to develop a three dimensional (3-D) porous bio-resorbable scaffold with a biomimetic pore structure. Osseous healing and integration with the surrounding tissue depends in part on new blood vessel formation within the porous structure. Since endothelial cells play a key role in angiogenesis (formation of new blood vessels from preexisting vasculature), the purpose of this study was to better understand human endothelial cell attachment, viability, growth, and phenotypic expression on sintered PLAGA microsphere scaffold. Scanning electron microscopy (SEM) examination showed cells attaching to the surface of microspheres and bridging the pores between the microspheres. Cell proliferation studies indicated that cell number increased during early stages and reached a plateau between days 10 and 14. Immunofluorescent staining for actin showed that cells were proliferating three dimensionally through the scaffolds while staining for PECAM-1 (platelet endothelial cell adhesion molecule) displayed typical localization at cell-cell- contacts. Gene expression analysis showed that endothelial cells grown on PLAGA scaffolds maintained their normal characteristic phenotype. The cell I proliferation and phenotypic expression were independent of scaffold pore architecture. These results demonstrate that PLAGA sintered microsphere scaffolds can support the growth and biological functions of human endothelial cells. The insights from this study should aid future. studies. aimed at enhancing angiogenesis in three dimesional tissue engineered scaffolds.