The secE gene encodes an integral membrane protein required for protein export in Escherichia coli.
The secE gene encodes an integral membrane protein required for protein export in Escherichia coli.
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secE 基因编码大肠杆菌中蛋白质输出所需的整合膜蛋白。
DOI：
10.1101/gad.3.7.1035
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发表时间：
1989
影响因子：
10.5
通讯作者：
Jon Beckwith
中科院分区：
文献类型：
--
作者：
P. Schatz;P. D. Riggs;A. Jacq;M. Fath;Jon Beckwith
采用secA-lacZ融合菌株的遗传筛选和选择过程反复产生了影响大肠杆菌蛋白质输出途径的四个基因的突变。这些基因分别是secA、secD、prlA/secY和secE。我们讨论了在广泛使用这种方法后未能找到新的sec基因的意义。其中一个基因，secE，已经被详细地描述了。从该基因的DNA序列和碱性磷酸酶与SecE蛋白的融合分析来看，我们认为它是一个13,600道尔顿的完整细胞质膜蛋白。本文和随附论文中的数据强烈表明，secE在大肠杆菌蛋白输出中起重要作用。
Genetic screening and selection procedures employing a secA-lacZ fusion strain repeatedly have yielded mutations in four genes affecting the protein export pathway of Escherichia coli. These genes are secA, secD, prlA/secY, and secE. We discuss the significance of the failure to find new sec genes after extensive use of this approach. One of the genes, secE, has been characterized in some detail. From the DNA sequence of the gene and analysis of alkaline phosphatase fusions to the SecE protein, we propose that it is a 13,600-dalton integral cytoplasmic membrane protein. The data presented here and in the accompanying paper strongly suggest that secE has an important role in E. coli protein export.