Nanorough titanium surfaces reduce adhesion of Escherichia coli and Staphylococcus aureus via nano adhesion points
Nanorough titanium surfaces reduce adhesion of Escherichia coli and Staphylococcus aureus via nano adhesion points
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DOI：
10.1016/j.colsurfb.2016.05.049
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发表时间：
2016-09-01
影响因子：
5.8
通讯作者：
Jandt, Klaus D.
中科院分区：
文献类型：
--
作者：
Luedecke, Claudia;Roth, Martin;Jandt, Klaus D.
微生物粘附在天然和合成材料表面是一个关键问题，例如在食品工业，污水处理，最重要的是在生物医学领域。目前，纳米材料表面结构控制微生物粘附的发展和进展需要对微生物-材料相互作用有更深入的了解。本研究旨在研究微生物-材料界面的纳米结构，并将其与微生物粘附动力学联系起来，作为钛表面纳米粗糙度的函数，以获得通过材料表面纳米粗糙度控制微生物粘附的新见解。与表面峰密度最高的2 nm物理气相沉积钛薄膜相比，表面峰密度最低的6 nm物理气相沉积钛薄膜上大肠杆菌和金黄色葡萄球菌的粘附力分别降低了55.6%和40.5%，差异有统计学意义（p < 0.05）。利用聚焦离子束（FIB）和扫描电镜（SEM）成像对微生物细胞进行横切，首次提供了对钛-微生物界面的直接洞察。高分辨率扫描电镜显示，表面峰是微生物细胞与材料表面初始接触的位点。在定性模型中，我们提出微生物在纳米级表面的初始粘附是由钛表面峰值密度通过纳米粘附点控制的。这一新的认识将有助于设计控制微生物粘附的材料表面。(C) 2016 Elsevier B.V.版权所有
Microbial adhesion to natural and synthetic materials surfaces is a key issue e.g. in food industry, sewage treatment and most importantly in the biomedical field. The current development and progress in nanoscale structuring of materials surfaces to control microbial adhesion requires an advanced understanding of the microbe-material-interaction. This study aimed to investigate the nanostructure of the microbe-material-interface and link it to microbial adhesion kinetics as function of titanium surface nanoroughness to gain new insight into controlling microbial adhesion via materials' surface nanoroughness. Adhesion of Escherichia coli and Staphylococcus aureus was statistically significantly reduced (p < 0.05) by 55.6 % and 40.5 %, respectively, on physical vapor deposited titanium thin films with a nanoroughness of 6 nm and the lowest surface peak density compared to 2 nm with the highest surface peak density. Cross-sectioning of the microbial cells with a focused ion beam (FIB) and SEM imaging provided for the first time direct insight into the titanium-microbe-interface. High resolution SEM micrographs gave evidence that the surface peaks are the loci of initial contact between the microbial cells and the material's surface. In a qualitative model we propose that the initial microbial adhesion on nanorough surfaces is controlled by the titanium surface peak density via nano adhesion points. This new understanding will help towards the design of materials surfaces for controlling microbial adhesion. (C) 2016 Elsevier B.V. All rights reserved.