A Flower Specific Calcineurin B-Like Molecule (CBL)-Interacting Protein Kinase (CIPK) Homolog in Tomato Cultivar Micro-Tom (Solanum lycopersicum L.)
A Flower Specific Calcineurin B-Like Molecule (CBL)-Interacting Protein Kinase (CIPK) Homolog in Tomato Cultivar Micro-Tom (Solanum lycopersicum L.)
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DOI：
10.4236/ajps.2012.36091
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发表时间：
2012-06
期刊：
影响因子：
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通讯作者：
T. Yuasa;Yushi Ishibashi;M. Iwaya‐Inoue
中科院分区：
文献类型：
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作者：
T. Yuasa;Yushi Ishibashi;M. Iwaya‐Inoue
与营养器官相比，高等植物的花器官和生殖器官对生物和非生物胁迫相对敏感。钙调神经磷酸酶B样分子（CBL）相互作用蛋白激酶（CIPK）参与了盐、干旱、低温等环境胁迫下的获得性耐受和适应。利用半定量RT-PCR技术对番茄Micro-Tom（Solanum lycopersicum L.）表明SlCIPK 2在花器官中特异表达。抗CIPK特异性抗体特异性识别重组SlCIPK 2，并在花中，特别是在雄蕊中以显著水平与CIPK相关多肽交叉反应。花特异性CIPK与微粒体紧密相关。重组SlCIPK 2的体外pull-down分析表明，SlCIPK 2与SlCBL和胁迫响应转录因子SlERF 7、SlCBF 1和SlAREB 1相互作用。本研究结果表明，花特异性CIPK基因SlCIPK 2可能通过CBLs参与番茄的钙信号转导，并可能通过雄蕊中的逆境应答转录因子介导番茄的逆境耐受。
Floral and reproductive organs of higher plants are relatively sensitive to biotic and abiotic stresses compared with the vegetative organs. Calcineurin B-like molecule (CBL) interacting protein kinase (CIPK) has appeared to be involved in acquired tolerance and acclimation under environmental stresses such as salinity, drought and chilling. Semi-quantitative RT-PCR using the vegetative and reproductive organs of tomato Micro-Tom (Solanum lycopersicum L.) at the various developmental stages indicated that SlCIPK2 was expressed specifically in the floral organ. An anti-CIPK specific antibody recognized the recombinant SlCIPK2 specifically and cross-reacted to a CIPK-related polypeptide at a significant level in flower, particularly in stamen. The flower specific CIPK was tightly associated with the microsomes. In vitro pull-down assay of the recombinant SlCIPK2 showed that SlCIPK2 interacts with SlCBLs and stress-responsive transcription factors, SlERF7, SlCBF1 and SlAREB1. The present data suggested that the flower-specific CIPK, SlCIPK2, was involved in calcium signaling in tomato via CBLs and stress tolerance possibly mediated by the stress-responsive transcription factors in stamen.