An innovative and user-friendly scoring system for standardised quantitative interpretation of the urine-based point-of-care strip test (POC-CCA) for the diagnosis of intestinal schistosomiasis: a proof-of-concept study
An innovative and user-friendly scoring system for standardised quantitative interpretation of the urine-based point-of-care strip test (POC-CCA) for the diagnosis of intestinal schistosomiasis: a proof-of-concept study
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DOI：
10.1016/j.actatropica.2019.105150
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发表时间：
2019-11-01
期刊：
影响因子：
2.7
通讯作者：
van Dam, Govert J.
中科院分区：
文献类型：
--
作者：
Casacuberta-Partal, Miriam;Hoekstra, Pytsje T.;van Dam, Govert J.
用于检测尿液中血吸虫循环阴极抗原 (POC-CCA) 的即时检测试纸条检测已被证明是一种用户友好且灵敏的替代粪便显微镜检查的方法，用于诊断曼氏血吸虫感染。然而，根据定义，测试的视觉评分取决于观察者，并引发有关定性解释的讨论，特别是在测试线往往较弱的低强度感染中。为了标准化视觉评分，开发和评估了一种用于 POC-CCA 盒半定量解释的创新方法，称为 G 分数。来自曼氏沙门氏菌流行区的尿液（n = 110）被用来评估这种新方法。将 POC-CCA 测试线与 G 分数进行视觉比较，即一系列包含不同强度喷墨打印条带的人工盒，以便按 1 至 10 的等级对 POC-CCA 测试线进行分级。在 G 分数和每克粪便中的卵数之间观察到显着的正相关性（Spearman 0.660，p < 0.001）。这项概念验证研究证明了 G 分数对于标准化 POC-CCA 尿液试纸条检测视觉评分的有用性。几个研究小组已经表示对他们的实地工作的 G 分数感兴趣。盒式磁带的进一步分发，特别是与参考标准结合使用时，将有助于更广泛的血吸虫病社区处理批次间差异和痕量读数解释等问题。
The point-of-care strip assay for the detection of the schistosome Circulating Cathodic Antigen (POC-CCA) in urine has shown to be a user-friendly and sensitive alternative to stool microscopy for the diagnosis of Schistosoma mansoni infections. However, visual scoring of the test is by definition observer dependent and leads to discussion about the qualitative interpretation, in particular in low intensity infections when test lines tend to be weak. In order to standardise visual scoring, an innovative approach for semi-quantitative interpretation of the POC-CCA cassettes, called G-scores, was developed and evaluated. Urines (n = 110) from a S. mansoni endemic area were used to evaluate this new approach. Test lines of the POC-CCA were visually compared against the G-scores, i.e. a series of artificial cassettes containing inkjet-printed strips of different intensities in order to grade the POC-CCA test line on a scale of 1 to 10. A significant positive correlation (Spearman 0.660, p < 0.001) was observed between G-scores and eggs per gram of faeces. This proof-of-concept study demonstrates the usefulness of the G-scores for standardising the visual scoring of the POC-CCA urine strip assay. Several research groups have already indicated an interest in the G-scores for their field work. Further distribution of the cassettes, in particular when provided in combination with reference standards, will assist the wider schistosomiasis community in dealing with issues like batch-to-batch differences and interpretation of trace readings.