Handling and detection of 25 amol of near-infrared dye deoxynucleotide conjugates by capillary electrophoresis with laser-induced fluorescence detection.
Handling and detection of 25 amol of near-infrared dye deoxynucleotide conjugates by capillary electrophoresis with laser-induced fluorescence detection.
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DOI：
10.1016/s0021-9673(03)00855-0
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发表时间：
2003-07
期刊：
影响因子：
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通讯作者：
Guo-dong Li;Jianxin Gao;Xiaojuan Zhou;O. Shimelis;R. Giese
中科院分区：
文献类型：
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作者：
Guo-dong Li;Jianxin Gao;Xiaojuan Zhou;O. Shimelis;R. Giese
Near-infrared dyes are attractive as labeling reagents to enhance sensitivity in trace analysis largely because background fluorescence is low in this spectral region. Here we demonstrate, towards a goal of detecting DNA adducts in small biological samples, that some near-infrared (IR) dye-labeled deoxynucleotides can be separated and detected with high sensitivity by capillary electrophoresis (CE)–laser-induced fluorescence detection (LIF) in a realistic way (handling detection limit of 25 amol) for near-IR dye-labeled deoxynucleotides. This detection limit is achieved by polarity-switching injection of 2.0 μl from a volume of 5.0 μl, in which the compounds are 5·10−12mol/l in 50% aqueous methanol. Although the adenine and cytosine-containing conjugates co-migrated, the other three (guanine, N2-ethylguanine and thymine) were resolved.
