Surface modification of cellulose nanocrystal with chitosan oligosaccharide for drug delivery applications
Surface modification of cellulose nanocrystal with chitosan oligosaccharide for drug delivery applications
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DOI：
10.1007/s10570-013-9954-y
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发表时间：
2013-08-01
期刊：
影响因子：
5.7
通讯作者：
Tam, Kam C.
中科院分区：
文献类型：
--
作者：
Akhlaghi, Seyedeh Parinaz;Berry, Richard C.;Tam, Kam C.
通过用壳寡糖（CSOS）修饰氧化纤维素纳米晶体（CNC）的表面，开发了一种基于两种最丰富的天然生物聚合物的新型药物递送系统。首先，使用2,2,6,6-四甲基哌啶-1-氧基自由基催化剂将CNC的伯醇部分选择性氧化为羧基。然后，使用 N-羟基琥珀酰亚胺和 1-乙基-3-(3-二甲基氨基丙基)-碳二亚胺作为偶联剂，通过碳二亚胺反应，CSOS 的氨基与氧化 CNC (CNC-OX) 上的羧酸基团发生反应。通过红外光谱、热重分析、电位滴定和 zeta 电位测量证实了 CSOS 成功接枝到 CNC-OX。接枝导致 CNC-OX 上的羧基转化率接近 90%，取代度为 0.26。 CNC-CSOS 纳米颗粒的结合效率为 21.5%，载药量为 14% w/w。使用药物选择性电极直接测量 CNC-CSOS 颗粒释放的盐酸普鲁卡因的浓度。在pH 8下研究了体外药物释放，纳米颗粒显示出长达1小时的快速释放，可用作透皮递送应用的生物相容性和可生物降解的药物载体。
A novel drug delivery system based on two of the most abundant natural biopolymers was developed by modifying the surface of oxidized cellulose nanocrystal (CNC) with chitosan oligosaccharide (CSOS). First, the primary alcohol moieties of CNC were selectively oxidized to carboxyl groups using the 2,2,6,6-tetramethylpiperidine-1-oxyl radical catalyst. The amino groups of CSOS were then reacted with carboxylic acid groups on oxidized CNC (CNC-OX) via the carbodiimide reaction using N-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide as coupling agents. Successful grafting of CSOS to CNC-OX was confirmed by infrared spectroscopy, thermogravimetry, potentiometric titration, and zeta potential measurements. The grafting resulted in a conversion of similar to 90 % carboxyl groups on CNC-OX and the degree of substitution was 0.26. CNC-CSOS nanoparticles showed a binding efficiency of 21.5 % and a drug loading of 14 % w/w. A drug selective electrode was used to directly measure the concentration of procaine hydrochloride released from CNC-CSOS particles. The in vitro drug release was studied at pH 8 and the nanoparticles revealed a fast release of up to 1 h, which can be used as biocompatible and biodegradable drug carriers for transdermal delivery applications.