Quantitative real-time PCR (qPCR) - based tool for detection and quantification of Cordyceps militaris in soil
Quantitative real-time PCR (qPCR) - based tool for detection and quantification of Cordyceps militaris in soil
复制标题
基于实时定量 PCR (qPCR) 的工具，用于检测和定量土壤中的蛹虫草
DOI：
10.1016/j.jip.2014.11.002
复制
发表时间：
2014
影响因子：
3.4
通讯作者：
Kamata N
中科院分区：
文献类型：
--
作者：
Saragih SA;Takemoto S;Hisamoto Y;Sato H;Fujii M;Kamata N
建立了一对特异性引物CM 2946 F/CM 3160 R的实时荧光定量PCR检测方法。以基因组DNA和高压灭菌土壤DNA提取物为标准曲线，测定了一定剂量C.军事中止。在采集的10个森林土壤样品中，有2个样品检测到了蛹虫草子实体。结果表明，该方法能有效地检测C.对土壤中的C.在不同的生态系统中。
A quantitative real-time PCR using a primer pair CM2946F/CM3160R was developed for specific detection and quantification ofCordycepsmilitarisfrom soil. Standard curves were obtained for genomic DNA and DNA extracts from autoclaved soil with a certain dose ofC. militarissuspension.C. militariswas detected from two forest soil samples out of ten that were collected when fruit bodies ofC. militariswere found. This method seemed effective in detection ofC. militarisin the soil and useful for rapid and reliable quantification ofC. militarisin different ecosystems.