A Fluorescent Polymeric Quantum Dot/ Aptamer Superstructure and Its Application for Imaging of Cancer Cells
A Fluorescent Polymeric Quantum Dot/ Aptamer Superstructure and Its Application for Imaging of Cancer Cells
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DOI：
10.1002/asia.201301676
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发表时间：
2014-05-01
影响因子：
4.1
通讯作者：
Qin, Yingqiang
中科院分区：
文献类型：
--
作者：
Jie, Guifen;Zhao, Yanbin;Qin, Yingqiang
本文利用分子工程技术制备了一种具有高强度荧光的聚合物量子点/适体超结构，并成功应用于癌细胞的荧光成像。聚合物上层结构由多个基于细胞的适体和高比例的量子点（QD）标记的DNA组成，利用适体的靶识别能力，通过多价效应增强细胞内化，以及聚合物偶联物的细胞破坏。重要的是，聚合物上层结构显示出越来越增强的荧光随着记录时间的推移，因此适合长期荧光细胞成像。量子点超结构独特而优异的荧光特性为开发聚合物量子点超结构铺平了道路，这些超结构有望应用于体内成像等领域。
In this work, a novel polymeric quantum dot/aptamer superstructure with a highly intense fluorescence was fabricated by a molecular engineering strategy and successfully applied to fluorescence imaging of cancer cells. The polymeric superstructure, which is composed of both multiple cell-based aptamers and a high ratio of quantum dot (QD)-labeled DNA, exploits the target recognition capability of the aptamer, an enhanced cell internalization through multivalent effects, and cellular disruption by the polymeric conjugate. Importantly, the polymeric superstructure exhibits an increasingly enhanced fluorescence with recording time and is thus suitable for long-term fluorescent cellular imaging. The unique and excellent fluorescence property of the QD superstructure paves the way for developing polymeric QD superstructures that hold promise for applications such as in vivo imaging.