Sublingual administration of Lactobacillus paracasei KW3110 inhibits Th2-dependent allergic responses via upregulation of PD-L2 on dendritic cells
Sublingual administration of Lactobacillus paracasei KW3110 inhibits Th2-dependent allergic responses via upregulation of PD-L2 on dendritic cells
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DOI：
10.1016/j.clim.2012.01.008
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发表时间：
2012-05-01
影响因子：
8.6
通讯作者：
Okamoto, Yoshitaka
中科院分区：
文献类型：
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作者：
Inamine, Ayako;Sakurai, Daijyu;Okamoto, Yoshitaka
乳酸菌在免疫调节治疗中具有潜力，但其临床疗效和机制尚不清楚。我们的目的是阐明热灭活副干酪乳杆菌KW3110和嗜酸乳杆菌L-92在胃内和舌下诱导的抗过敏免疫反应。KW3110菌株（而不是L-92菌株）增强了卵清蛋白（OVA）诱导的小鼠树突状细胞（dc）中CCR-7和PD-L2的表达，并在体外与来自DO11.10转基因小鼠的th2倾斜CD4(+) T细胞共培养时强烈抑制IL-5和IL-13的产生。低剂量KW3110（而非L-92）舌下给药OVA致敏小鼠选择性抑制血清IgE产生和颈部淋巴结Th2细胞因子表达，并显著改善体内OVA激发后的症状。KW3110可能通过增强CCR-7和PD-L2的表达，加速DC向区域淋巴结的迁移，抑制Th2细胞因子的产生。因此，舌下给药KW3110可能有效减少过敏性炎症。(C) 2012爱思唯尔公司版权所有。
Lactic acid bacteria have potential in immunomodulation therapy, but their clinical efficacy and underlying mechanisms are unclear. We aimed to clarify the anti-allergic immune responses induced by intragastric and sublingual administration of heat-killed Lactobacillus paracasei KW3110 and Lactobacillus acidophilus L-92. The KW3110 strain (but not the L-92 strain) enhanced ovalbumin (OVA)-induced expression of CCR-7 and PD-L2 in murine dendritic cells (DCs), and strongly inhibited IL-5 and IL-13 production in vitro in co-cultures with Th2-skewed CD4(+) T cells from DO11.10 transgenic mice. Sublingual administration of low-dose KW3110 (but not L-92) to OVA-sensitized mice selectively suppressed serum IgE production and Th2 cytokine expression in cervical lymph nodes, and significantly improved symptoms after OVA provocation in vivo. KW3110 probably accelerates DC migration into the regional lymph nodes and inhibits Th2 cytokine production through enhanced CCR-7 and PD-L2 expression. Thus, sublingual KW3110 administration may be effective in reducing allergic inflammation. (C) 2012 Elsevier Inc. All rights reserved.