Extensive somatic mitochondrial mutations in primary prostate cancer using laser capture microdissection.
Extensive somatic mitochondrial mutations in primary prostate cancer using laser capture microdissection.
复制标题
使用激光捕获显微切割在原发性前列腺癌中进行广泛的体细胞线粒体突变。
作者：
Junjian Z. Chen;N. Gokden;G. Greene;P. Mukunyadzi;F. Kadlubar
前列腺癌是美国男性癌症死亡的第二大原因，但导致前列腺癌发生的确切分子事件尚不清楚。我们使用激光捕获显微切割从前列腺癌及其浸润前病变中分离出组织学定义的细胞群，并对线粒体基因组（一种敏感的细胞质DNA）进行遗传分析。在线粒体DNA的控制区（置换环）发现了极高的体细胞突变发生率（90%的乳腺癌切除术标本）。病变相关突变的大量诱导表明，在前列腺癌及其浸润前病变中存在活跃的线粒体突变。这些突变的检查提供了新的见解前列腺癌遗传学和揭示前列腺肿瘤病变的体细胞突变的独特模式。
Prostate cancer is the second leading cause of cancer deaths among men in the United States,but the precise molecular events leading to prostate carcinogenesis are not well understood. We isolated histologically defined cell populations from prostate cancer and its preinvasive lesions using laser capture microdissection, and performed genetic analysis on the mitochondrial genome, a sensitive cytoplasmic DNA. An extremely high incidence of somatic mutation (90% of prostatectomy cancer specimens) was found in the control region (the displacement loop) of mitochondrial DNA. The massive induction of lesion-associated mutations suggests active mitochondrial mutagenesis in both prostate cancer and its preinvasive lesions. Inspection of these mutations provides new insights into prostate cancer genetics and reveals unique patterns of somatic mutations in prostatic neoplastic lesions.
DOI：
10.1073/pnas.94.2.514
发表时间：
1997-01-21
影响因子：
11.1
作者：
Yakes, FM;VanHouten, B
通讯作者：
VanHouten, B
影响因子：
56.9
作者：
Fliss, MS;Usadel, H;Sidransky, D
通讯作者：
Sidransky, D
DOI：
10.1016/s0027-5107(99)00206-7
发表时间：
2000-02-14
影响因子：
2.3
作者：
Jackson, AL;Loeb, LA
通讯作者：
Loeb, LA