MDR‐like ABC transporter AtPGP4 is involved in auxin‐mediated lateral root and root hair development
MDR‐like ABC transporter AtPGP4 is involved in auxin‐mediated lateral root and root hair development
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DOI：
10.1016/j.febslet.2005.08.061
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发表时间：
2005-10
期刊：
影响因子：
3.5
通讯作者：
Diana Santelia;Vincent Vincenzetti;E. Azzarello;L. Bovet;Y. Fukao;Petra Düchtig;S. Mancuso;E. Martinoia;Markus Geisler
中科院分区：
文献类型：
--
作者：
Diana Santelia;Vincent Vincenzetti;E. Azzarello;L. Bovet;Y. Fukao;Petra Düchtig;S. Mancuso;E. Martinoia;Markus Geisler
以前的数据表明，MDR/PGP样ABC转运蛋白参与植物激素生长素的运输，最近，AtPGP 1已被证明催化生长素的主要活性出口。在这里，我们表明，相关的亚型AtPGP 4主要是在早期根的发展。AtPGP 4功能丧失的植物揭示了增强的侧根起始和根毛长度，这两者都已知是在生长素的控制下。此外，atpgp 4植物显示出对生长素和生长素转运抑制剂NPA的敏感性改变。最后，突变体根揭示了升高的游离生长素水平和降低的生长素运输能力。这些结果与酵母生长测定表明，AtPGP 4在生长素运输过程中控制侧根和根毛发育的直接参与。
Previous data have suggested an involvement of MDR/PGP-like ABC transporters in transport of the plant hormone auxin and, recently, AtPGP1 has been demonstrated to catalyze the primary active export of auxin. Here we show that related isoform AtPGP4 is expressed predominantly during early root development. AtPGP4 loss-of-function plants reveal enhanced lateral root initiation and root hair lengths both known to be under the control of auxin. Further, atpgp4 plants show altered sensitivities toward auxin and the auxin transport inhibitor, NPA. Finally, mutant roots reveal elevated free auxin levels and reduced auxin transport capacities. These results together with yeast growth assays suggest a direct involvement of AtPGP4 in auxin transport processes controlling lateral root and root hair development.