COMPARISON OF GRAM STAIN, DNA PROBE, AND CULTURE FOR THE IDENTIFICATION OF SPECIES OF MOBILUNCUS IN FEMALE GENITAL SPECIMENS
COMPARISON OF GRAM STAIN, DNA PROBE, AND CULTURE FOR THE IDENTIFICATION OF SPECIES OF MOBILUNCUS IN FEMALE GENITAL SPECIMENS
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DOI：
10.1093/infdis/152.1.74
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发表时间：
1985-01-01
影响因子：
6.4
通讯作者：
HOLMES, KK
中科院分区：
文献类型：
--
作者：
ROBERTS, MC;HILLIER, SL;HOLMES, KK
以临终关怀病人、大学生和性病门诊就诊者的标本为样本，比较DNA探针(全染色体细菌DNA)、培养和革兰氏染色三种方法检测女性生殖器标本中移行线虫的种类。培养纯化和物种形成平均需要37天才能完成，而DNA探针分析需要5天。革兰氏染色也很快，但不允许物种形成。DNA探针与常规生化方法鉴定菌种的符合率为100%。无论哪种方法，仅革兰氏染色就能检出90%的阳性样本，而培养法能检出77%-83%，DNA探针能检出52%-83%。
The detection of species of Mobiluncus in female genitial specimens by DNA probe (with whole-chromosomal bacterial DNA), culture, and gram stain were compared by using specimens obtained from hosptial patients, college students, and women attending a sexually transmitted disease clinic. Culture purification and speciation required an average of 37 days to complete, whereas the DNA-probe assay required 5 days. Gram stain was also rapid but did not allow speciation. There was 100% correlation between species identification by DNA probe and by conventional biochemical tests. Gram stain alone detected 90% of the samples that were postive by any method, whereas culture detected 77%-83% and DNA probe detected 52%-83%.