Evolution of transposable elements: an IS10 insertion increases fitness in Escherichia coli.
Evolution of transposable elements: an IS10 insertion increases fitness in Escherichia coli.
复制标题
转座元件的进化：IS10 插入增加了大肠杆菌的适应性。
DOI：
10.1093/oxfordjournals.molbev.a040356
复制
发表时间：
1985
影响因子：
10.7
通讯作者：
McBroom,SM
中科院分区：
文献类型：
--
作者：
Chao,L;McBroom,SM
Strains of Escherichia coli carrying Tn10, a transposon consisting of two IS10 insertion sequences flanking a segment encoding for a tetracycline-resistance determinant, gain a competitive advantage in chemostat cultures. All Tn10-bearing strains that increase in frequency during competition have a new IS10 insertion that is found in the same location in the genome of those strains. We mapped, by a gradient of transmission, the position of the new IS10 insertion. We examined 11 isolates whose IS10 insertion was deleted by recombinational crossing-over, and in all cases the competitive fitness of the isolates was decreased. These results show that the IS10-generated insertion increases fitness in chemostat cultures. We named the insertion fit::IS10 and suggest that transposable elements may speed the rate of evolution by promoting nonhomologous recombination between preexisting variations within a genome and thereby generating adaptive variation.
