Expansion of innate CD5pos B cells expressing high levels of CD81 in hepatitis C virus infected liver
Expansion of innate CD5pos B cells expressing high levels of CD81 in hepatitis C virus infected liver
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DOI：
10.1016/s0168-8278(03)00075-8
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发表时间：
2003-05-01
影响因子：
25.7
通讯作者：
O'Farrelly, C
中科院分区：
文献类型：
--
作者：
Curry, MP;Golden-Mason, L;O'Farrelly, C
背景/目的：丙型肝炎病毒（HCV）与自身抗体增加、混合冷球蛋白血症、非霍奇金B细胞淋巴瘤和外周血先天（CD5(pos)） B细胞增加有关，提示B淋巴细胞在HCV感染的发病机制中起作用。方法：采用流式细胞术检测慢性HCV感染中CD5(pos) B细胞水平和CD81共表达。采用PCR检测病毒载量。结果：我们发现，与正常肝脏（8%）相比，纤维化评分低于II期（39%，占总B细胞的%，P = 0.002）和终末期HCV肝硬化（20%,P < 0.05）的HCV感染患者肝脏中的先天B细胞扩增。与常规B细胞相比，外周血CD5(pos) B细胞中CD81（一种信号转导分子和假定的HCV受体）的表达显著增加（P = 0.0001）。在hcv感染个体的肝脏中，CD5(pos) B细胞上CD81水平的升高更为显著。然而，CD5(pos)CD81(High) B细胞和CD5(negative)CD81(Low) B细胞的病毒载量没有显著差异。结论：先天B细胞CD81的表达增加，在慢性HCV感染患者的肝脏和外周血中扩增，提示在慢性HCV感染中病毒特异性激活和克隆增殖中起作用。(C) 2003年欧洲肝脏研究协会。Elsevier Science B.V.版权所有。
Background/Aims: Association of hepatitis C virus (HCV) with increased autoantibodies, mixed cryoglobulinaemia, non-Hodgkin's B-cell lymphoma and increased peripheral innate (CD5(pos)) B cells suggests a role for B-lymphocytes in the pathogenesis of HCV-infection.Methods: Flow cytometry was used to estimate CD5(pos) B cell levels and CD81 co-expression in chronic HCV infection. Viral load was assessed using PCR.Results: We demonstrate expansion of innate B cells in HCV-infected liver from patients with fibrosis score less than stage II (39%, % of total B cells, P = 0.002) and end stage HCV cirrhosis (20%, P < 0.05) compared with normal liver (8%). Expression of CD81, a signal transducing molecule and putative HCV receptor, was significantly increased on peripheral blood CD5(pos) B cells compared with conventional B cells (P = 0.0001). Higher levels of CD81 on CD5(pos) B cells were more dramatic in the liver of HCV-infected individuals. However, no significant difference was observed in the viral load of CD5(pos)CD81(High) B cells and CD5(neg)CD81(Low) B cells.Conclusions: Increased expression of CD81 on innate B cells, a population that is expanded in the livers and peripheral blood of chronic HCV-infected patients, suggests a role in viral specific activation and clonal proliferation in chronic HCV infection. (C) 2003 European Association for the Study of the Liver. Published by Elsevier Science B.V. All rights reserved.