Artifact-free and high-temporal-resolution in vivo opto-electrophysiology with microLED optoelectrodes
Artifact-free and high-temporal-resolution in vivo opto-electrophysiology with microLED optoelectrodes
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利用微LED光电极实现无伪影和高时间分辨率的体内光电生理学
DOI：
10.1038/s41467-020-15769-w
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发表时间：
2020-04-28
影响因子：
16.6
通讯作者：
Yoon, Euisik
中科院分区：
文献类型：
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作者：
Kim, Kanghwan;Voroslakos, Mihaly;Yoon, Euisik
体内细胞外记录和基因工程辅助光刺激的结合是研究神经元回路的有力工具。复杂神经回路的精确分析需要多个细胞大小的光源和记录电极的高密度集成。然而，高密度集成不可避免地引入了刺激伪影。我们提出了最小刺激伪影（miniSTAR） mu LED光电电极，可以有效消除刺激伪影。具有屏蔽层的多金属层结构有效地抑制了刺激信号的电容耦合。大量掺硼的硅衬底抑制了LED照明引起的光电效应。通过瞬态刺激脉冲整形，我们将miniSTAR mu LED光电电极上的刺激误差降低到50 mu V-pp以下，远小于典型的峰值检测阈值，光刺激强度为50mWmm（-2）。我们展示了高时间分辨率(
The combination of in vivo extracellular recording and genetic-engineering-assisted optical stimulation is a powerful tool for the study of neuronal circuits. Precise analysis of complex neural circuits requires high-density integration of multiple cellular-size light sources and recording electrodes. However, high-density integration inevitably introduces stimulation artifact. We present minimal-stimulation-artifact (miniSTAR) mu LED optoelectrodes that enable effective elimination of stimulation artifact. A multi-metal-layer structure with a shielding layer effectively suppresses capacitive coupling of stimulation signals. A heavily boron-doped silicon substrate silences the photovoltaic effect induced from LED illumination. With transient stimulation pulse shaping, we reduced stimulation artifact on miniSTAR mu LED optoelectrodes to below 50 mu V-pp, much smaller than a typical spike detection threshold, at optical stimulation of >50mWmm(-2) irradiance. We demonstrated high-temporal resolution (