Transcriptional interactions between the pannier isoforms and the cofactor U-shaped during neural development in Drosophila
Transcriptional interactions between the pannier isoforms and the cofactor U-shaped during neural development in Drosophila
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DOI：
10.1016/j.mod.2010.08.002
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发表时间：
2010-09-01
影响因子：
2.6
通讯作者：
Ramain, Philippe
中科院分区：
文献类型：
--
作者：
Fromental-Ramain, Catherine;Taquet, Nathalie;Ramain, Philippe
黑腹果蝇pannier（pnr）基因编码属于加塔转录因子家族的两种异构体。同种型在翅盘中共享表达结构域，在那里它们在前神经性无毛/盾板（ac/sc）基因的调节期间表现出不同的功能。我们先前确定了pnr基因座中的两个区域，它们驱动转基因株系中的报告基因表达，其模式概括了两种亚型表达的基本特征。在这里，我们确定了驱动异构体表达的启动子区域，表明pnr-α调控序列接近转录起始位点，而pnr-β表达需要近端和远端调控元件之间的功能相互作用。我们发现，报告基因表达所必需的启动子结构域也介导Pnr-β的自动调节和Pnr-β对Pnr-α的抑制。辅因子U形（Ush），这是已知的下调Pnr的功能，在胸部图案转录后，此外抑制pnr-b所需的ac/sc激活。此外，Ush负调控其自身的表达，而pnr亚型正调控ush。我们的研究揭示了pnr亚型和辅助因子Ush之间复杂的转录相互作用，这可能对前神经表达和胸部图案的调节很重要。(C)2010爱思唯尔爱尔兰有限公司版权所有。
The pannier (pnr) gene of Drosophila melanogaster encodes two isoforms that belong to the family of GATA transcription factors. The isoforms share an expression domain in the wing discs where they exhibit distinct functions during regulation of the proneural achaete/scute (ac/sc) genes. We previously identified two regions in the pnr locus that drive reporter expression in transgenic lines in patterns that recapitulate the essential features of expression of the two isoforms. Here, we identify promoter regions driving isoform expression, showing that pnr-alpha regulatory sequences are close to the transcription start site while pnr-beta expression requires functional interactions between proximal and distal regulatory elements. We find that the promoter domains necessary for reporter expression also mediate autoregulation of Pnr-beta and repression of pnr-alpha by Pnr-beta. The cofactor U-shaped (Ush), which is known to down-regulate the function of Pnr during thorax patterning postranscriptionally, in addition represses pnr-b required for ac/sc activation. Moreover, Ush negatively regulates its own expression, while the pnr isoforms positively regulate ush. Our study uncovers complex transcriptional interactions between the pnr isoforms and the cofactor Ush that may be important for regulation of proneural expression and thorax patterning. (C) 2010 Elsevier Ireland Ltd. All rights reserved.