Fabricating autologous tissue to engineer artificial nerve
Fabricating autologous tissue to engineer artificial nerve
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DOI：
10.1002/micr.21740
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发表时间：
2002-01-01
期刊：
影响因子：
2.1
通讯作者：
Chen, ZR
中科院分区：
文献类型：
--
作者：
Cheng, B;Chen, ZR
本研究报道了用组织工程学方法成功构建人工神经。将雪旺细胞培养2周，接种于聚明胶910支架上，涂布鼠尾胶和层粘连蛋白生物膜。用光学显微镜、扫描和透射电子显微镜观察支架对雪旺细胞的吸附作用以及雪旺细胞的生长和迁移情况。雪旺细胞能够在聚乳蛋白910纤维上迁移和增殖。雪旺细胞分布均匀，并形成一条邦纳带，雪旺细胞在该带上产生较多的基质。生物膜上的雪旺细胞也生长良好。我们利用已建立的兔周围神经再生模型，研究了组织工程导管在体内的作用。8周时，远端神经残端可见轴突再生。成体雪旺细胞可在包被纤维和生物膜上生成。含雪旺细胞的三维支架具有人工神经的基本特征。这些发现将为构建组织工程化人工神经修复长神经缺损提供一种实用的方法。(C)2002年Wiley-Liss，Inc.
This study reports on the successful fabrication of artificial nerves with tissue engineering methods. Schwann cells were cultured for 2 weeks, seeded on polyglactin 910 scaffolds, and biomembrane-coated with rat-tail glue and laminin. Observation of the scaffolds' adsorptivity to Schwann cells, and of the growth and migration of Schwann cells, was made using a light microscope, and by scanning and transmission electron microscopy. The Schwann cells were able to migrate and proliferate on the polyglactin 910 fiber. Schwann cells were well-distributed, and formed a Bungner band on which the Schwann cells produced more matrices. Schwann cells on the biomembrane also grew well. We investigated the role of the tissue engineering conduit guide in vivo, using an established rabbit peripheral nerve regeneration model. At 8 weeks, axonal regeneration was observed in the distal nerve stump. Adult Schwann cells can be produced on the coated fiber and the biomembrane. Three-dimensional scaffolds with Schwann cells had the basic characteristics of the artificial nerve. These findings will provide a practical method for fabricating tissue-engineered artificial nerve to repair long nerve defects. (C) 2002 Wiley-Liss, Inc.