The ERF transcription factor MdERF38 promotes drought stress-induced anthocyanin biosynthesis in apple
The ERF transcription factor MdERF38 promotes drought stress-induced anthocyanin biosynthesis in apple
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ERF转录因子MdERF38促进干旱胁迫诱导的苹果花青素生物合成
DOI：
10.1111/tpj.14555
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发表时间：
2019-11-10
期刊：
影响因子：
7.2
通讯作者：
Hao, Yu-Jin
中科院分区：
文献类型：
--
作者：
An, Jian-Ping;Zhang, Xiao-Wei;Hao, Yu-Jin
干旱胁迫诱导多种植物花青素合成，但其分子机制尚不清楚。乙烯响应因子（ERFs）在植物生长和各种逆境响应中起关键作用，包括影响花青素的生物合成。在这里，我们鉴定了一个ERF蛋白MdERF38，它参与了干旱胁迫诱导的花青素生物合成。生化和分子分析表明，MdERF38与花青素生物合成的正调节因子MdMYB1相互作用，促进MdMYB1与其靶基因结合。因此，MdERF38促进了干旱胁迫下花青素的生物合成。此外，我们发现MdBT2是花青素生物合成的负调节因子，通过加速MdERF38蛋白的降解来降低MdERF38促进的花青素生物合成。总之，我们的数据提供了干旱胁迫诱导花青素生物合成的机制，该机制涉及MdERF38在转录和翻译后水平的动态调节。
Drought stress induces anthocyanin biosynthesis in many plant species, but the underlying molecular mechanism remains unclear. Ethylene response factors (ERFs) play key roles in plant growth and various stress responses, including affecting anthocyanin biosynthesis. Here, we characterized an ERF protein, MdERF38, which is involved in drought stress-induced anthocyanin biosynthesis. Biochemical and molecular analyses showed that MdERF38 interacted with MdMYB1, a positive modulator of anthocyanin biosynthesis, and facilitated the binding of MdMYB1 to its target genes. Therefore, MdERF38 promoted anthocyanin biosynthesis in response to drought stress. Furthermore, we found that MdBT2, a negative modulator of anthocyanin biosynthesis, decreased MdERF38-promoted anthocyanin biosynthesis by accelerating the degradation of the MdERF38 protein. In summary, our data provide a mechanism for drought stress-induced anthocyanin biosynthesis that involves dynamic modulation of MdERF38 at both transcriptional and post-translational levels.