Co-operation between neural and myogenic mechanisms in the control of distension-induced peristalsis in the mouse small intestine
Co-operation between neural and myogenic mechanisms in the control of distension-induced peristalsis in the mouse small intestine
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DOI：
10.1111/j.1469-7793.1998.843bv.x
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发表时间：
1998-02-01
影响因子：
5.5
通讯作者：
Der-Silaphet, T
中科院分区：
文献类型：
--
作者：
Huizinga, JD;Ambrous, K;Der-Silaphet, T
1.在扩张诱导的肠梗阻模型中研究了肠运输的肌源性和神经控制。比较对照小鼠和W/W-v小鼠的电活动、机械活动和内容物流出，W/W-v小鼠缺乏与奥尔巴赫丛相关的Cajal间质细胞。扩张引起的运动活动增加，由于在控制和W/W-v小鼠肠神经的刺激周期性的外观。兴奋主要由胆碱能神经传递，而周期性抑制则由神经元型一氧化氮介导.在对照组小鼠中，流出是由传播慢波活动驱动的，并且仅在反口方向上。只有当慢波携带足够的动作电位，通过直接刺激肌肉组织或扩张诱导的神经介导激活引起相位性管腔内压力增加大于或等于1 cmH（2）O时，才会发生流出。在W/W-v小鼠流出与传播的动作电位，发生由于神经刺激或直接肌肉刺激。动作电位的传播和流出发生在口侧和背侧两个方向。总之，在对照组和W/W-v小鼠中，扩张通过刺激肠神经系统诱导周期性运动活动。腔内内容物在这种运动活动之前没有移动。相反，在整个节段同时发生的活动期间内，脉动流出由控制组织中具有叠加动作电位的个体传播慢波和W/W-v小鼠中缺乏Cajal间质细胞的传播动作电位来引导。
1. Myogenic and neural control of intestinal transit were investigated in a model of distension-induced peristalsis. A comparison was made between the electrical and mechanical activities and outflow of contents observed in control mice and in W/W-v mice, which lack the interstitial cells of Cajal associated with Auerbach's plexus.2. Distension caused a periodic appearance of increased motor activity due to stimulation of enteric nerves in both control and W/W-v mice. Excitation was primarily delivered by cholinergic nerves, whereas periodic inhibition was mediated by neuronal nitric oxide.3. In control mice, outflow was driven by propagating slow-wave activity and was only in the aboral direction. Outflow only occurred when slow waves carried sufficient action potentials to cause phasic intraluminal pressure increases of greater than or equal to 1 cmH(2)O through direct stimulation of the musculature or by distension-induced neurally mediated activation.4. In W/W-v mice outflow was associated with propagating action potentials that occurred due to either neural stimulation or direct muscle stimulation. Action potential propagation and outflow occurred in both oral and aboral directions.5. In summary, in both control and W/W-v mice, distension induced periodic motor activity through stimulation of the enteric nervous system. Intraluminal contents were not moved in front of such motor activity. Rather, within such periods of activity that occurred concurrently throughout an entire segment, pulsatile outflow was directed by individual propagating slow waves with superimposed action potentials in control tissue, and by propagating action potentials in W/W-v mice, which lack interstitial cells of Cajal.