Polymethylmethacrylate and Titanium Alloy Particles Activate Peripheral Monocytes during Periprosthetic Inflammation and Osteolysis
Polymethylmethacrylate and Titanium Alloy Particles Activate Peripheral Monocytes during Periprosthetic Inflammation and Osteolysis
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DOI：
10.1002/jor.21287
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发表时间：
2011-05-01
影响因子：
2.8
通讯作者：
Wooley, Paul H.
中科院分区：
文献类型：
--
作者：
Yang, Shang-You;Zhang, Kai;Wooley, Paul H.
我们使用SCID-hu无菌性松动模型研究了颗粒PMMA或钛合金、患者血液单核细胞和假体周围组织的相互作用。将假体周围组织和因松动而进行翻修手术时获得的骨碎片移植到SCID小鼠的肌肉中。从相同供体分离的外周血单核细胞（PBMC）进行荧光标记，并在腹腔内注射前与PMMA或Ti-6Al-4V颗粒共培养。具有假体周围组织或非炎性韧带异种移植物的对照小鼠接受幼稚PBMC输注。在PBMC输注后2周将小鼠安乐死。人组织在SCID小鼠中接受良好。输注的荧光标记的PBMC在移植的假体周围组织中显著积聚。多核破骨细胞样细胞常见于取出的异种移植物组织中，局灶性骨侵蚀普遍存在。与未经处理的PBMC动物相比，在用PMMA和Ti激发的PBMC输注的小鼠中，异种移植物内的总细胞密度和CD 68+细胞显著增加（p < 0.05）。免疫组织化学染色在PMMA或Ti刺激的单核细胞输注组的异种移植物中鉴定出更强的阳性IL-1和TNF染色（p < 0.05）。在两个活化的PBMC组中在骨碎片周围发现TRAP+细胞，尽管在PMMA激发组中比在钛组中显著更多聚集的TRAP+细胞（p < 0.05）。MicroCT评估证实与活化的单核细胞/破骨细胞相互作用的芯片中的骨矿物质密度显著降低。总之，PMMA或钛颗粒容易激活外周单核细胞，并促进细胞运输到含碎片的假体组织。颗粒激发的PBMC参与并促进局部炎症过程、破骨细胞生成和骨吸收。(C)2010骨科研究学会。由威利期刊公司出版J Orthop Res 29：781-786，2011
We investigated the interactions of particulate PMMA or titanium alloy, patient blood monocytes, and periprosthetic tissues using a SCID-hu model of aseptic loosening. Periprosthetic tissues and bone chips obtained at revision surgery for loosening were transplanted into muscles of SCID mice. Peripheral blood monocytes (PBMCs) isolated from the same donors were fluorescently labeled and co-cultured with PMMA or Ti-6Al-4V particles before intraperitoneal injection. Control mice with periprosthetic tissue or non-inflammatory ligament xenografts received naive PBMCs transfusion. Mice were euthanized 2 weeks after PBMC transfusion. The human tissues were well accepted in SCID mice. Transfused fluorescent-labeled PBMCs were markedly accumulated in transplanted periprosthetic tissues. Multinucleated osteoclast-like cells were commonly seen within retrieved xenograft tissue, and focal bone erosions were ubiquitous. Total cell densities and CD68+ cells within the xenograft were significantly increased in mice transfused with PMMA and Ti-provoked PBMCs compared to the nayve PBMC animals (p < 0.05). Immunohistochemical staining identified much stronger positive IL-1 and TNF stains in xenografts from either PMMA or Ti-stimulated monocytes transfusion groups (p < 0.05). TRAP+ cells were found around bone chips in both activated-PBMCs groups, although markedly more aggregated TRAP+ cells in the PMMA-challenged group than in the titanium group (p < 0.05). MicroCT assessment confirmed the significant decrease of bone mineral density in chips interacted with activated-monocytes/ osteoclasts. In conclusion, PMMA or titanium particles readily activate peripheral monocytes and promote the cell trafficking to the debris-containing prosthetic tissues. Particles-provoked PBMCs participated in and promoted the local inflammatory process, osteoclastogenesis, and bone resorption. (C) 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29: 781-786, 2011