Incorporation of a FRET Pair into a Riboswitch RNA to Measure Mg(2+) Concentration and RNA Conformational Change in Cell.
Incorporation of a FRET Pair into a Riboswitch RNA to Measure Mg(2+) Concentration and RNA Conformational Change in Cell.
复制标题
将 FRET 对掺入核糖开关 RNA 中以测量细胞中 Mg2 浓度和 RNA 构象变化
DOI：
10.3390/ijms23031493
复制
发表时间：
2022-01-27
影响因子：
5.6
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Xue Y;Liu Y
Riboswitches are natural biosensors that can regulate gene expression by sensing small molecules. Knowledge of the structural dynamics of riboswitches is crucial to elucidate their regulatory mechanism and develop RNA biosensors. In this work, we incorporated the fluorophore, Cy3, and its quencher, TQ3, into a full-length adenine riboswitch RNA and its isolated aptamer domain to monitor the dynamics of the RNAs in vitro and in cell. The adenine riboswitch was sensitive to Mg2+ concentrations and could be used as a biosensor to measure cellular Mg2+ concentrations. Additionally, the TQ3/Cy3-labeled adenine riboswitch yielded a Mg2+ concentration that was similar to that measured using a commercial assay kit. Furthermore, the fluorescence response to the adenine of the TQ3/Cy3-labeled riboswitch RNA was applied to determine the proportions of multiple RNA conformational changes in cells. The strategy developed in this work can be used to probe the dynamics of other RNAs in cells and may facilitate the developments of RNA biosensors, drugs and engineering.
登录
查看更多内容
DOI：
10.1126/science.1207339
发表时间：
2011-07-29
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Paige JS;Wu KY;Jaffrey SR
通讯作者：
Jaffrey SR
腺嘌呤感应核糖开关适体结构域的金属离子结合和金属离子诱导折叠。
影响因子：
14.9
作者：
Noeske J;Schwalbe H;Wöhnert J
通讯作者：
Wöhnert J
DOI：
10.1002/anie.201806482
发表时间：
2019-01-28
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Neubacher S;Hennig S
通讯作者：
Hennig S
影响因子：
64.8
作者：
Reining, Anke;Nozinovic, Senada;Schwalbe, Harald
通讯作者：
Schwalbe, Harald
影响因子：
14.8
作者：
Ding, Yiliang;Kwok, Chun Kit;Assmann, Sarah M.
通讯作者：
Assmann, Sarah M.