Immortalization of inbred rabbit keratinocytes from a Shope papilloma and tumorigenic transformation of the cells by EJ-ras.
Immortalization of inbred rabbit keratinocytes from a Shope papilloma and tumorigenic transformation of the cells by EJ-ras.
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来自 Shope 乳头状瘤的近交兔角质形成细胞的永生化和 EJ-ras 对细胞的致瘤转化。
DOI：
10.1016/s0304-3835(96)04415-1
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发表时间：
1996
期刊：
影响因子：
9.7
通讯作者：
Kreider,JW
中科院分区：
文献类型：
--
作者：
Peng,X;Lang,CM;Kreider,JW
从棉尾兔乳头瘤病毒（CRPV）感染的近交系兔皮肤诱导的乳头瘤中，建立了角质形成细胞永活细胞系SPG1-3。细胞在培养中已经达到60代，并且仍然生长良好，但它们在胸腺小鼠中不是致瘤性的。虽然CRPV DNA在源自单一角质形成细胞的乳突瘤细胞系中作为染色体外片段存在，但在细胞中未检测到CRPV DNA。用EJ-ras转染SPG1-3细胞，建立SPG1-3EJ、SPG1-3EJ1和SPG1-3EJ2三个亚细胞系。所有三个亚系都含有EJ-ras DNA和一个1.2 kb的EJ-ras转录本，它们在胸腺小鼠中具有恶性致瘤性。这些数据表明，CRPV基因组及其表达可能对肿瘤的发生和维持至关重要，但对维持肿瘤来源细胞的永生化并不重要。此外，一些致癌基因如EJ-ras可能在永生化细胞的致瘤性和恶性转化中发挥重要作用。这些来源于近交系兔皮肤的细胞系，通过转染CRPV基因并连续移植到近交系兔体内，研究宿主对病毒致瘤潜能的免疫反应，可以为更好地了解乳头瘤病毒相关肿瘤进展的致瘤过程提供一个有用的体外系统。
An immortalized cell line of keratinocytes, named SPG1-3, was established from a papilloma induced from cottontail rabbit papillomavirus (CRPV)-infected inbred rabbit skin. The cells have reached 60 passages in culture and are still growing well, but they are not tumorigenic in athymic mice. Although CRPV DNA was present as extrachromosomal episomes in the papilloma from which the cell line was derived from a single colony of keratinocytes, there was no CRPV DNA detectable in the cells. Three sub-cell lines of SPG1-3EJ, SPG1-3EJ1 and SPG1-3EJ2 were then established from the EJ-ras transfected SPG1-3 cells. All of the three sub-lines contained both EJ-ras DNA and a 1.2 kb transcript of EJ-ras, and they are malignantly tumorigenic in athymic mice. These data indicate that CRPV genome and its expression might be essential for the initiation and maintenance of neoplasia, but not for the maintenance of immortalization of the tumor-derived cells. In addition, some oncogenes such as EJ-ras may play an essential role in tumorigenic and malignant conversion of the immortalized cells. These cell lines derived from inbred rabbit skin may provide a useful in vitro system for better understanding of the oncogenic processes of papillomavirus-involved neoplastic progression by transfecting the cells with CRPV genes and serial transplantation to the inbred rabbits for studying host immune responses to the viral oncogenic potential.