Utility of the Three-Dimensional Cultured Human Skin Model as a Tool to Evaluate Skin Permeation of Drugs
Utility of the Three-Dimensional Cultured Human Skin Model as a Tool to Evaluate Skin Permeation of Drugs
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三维培养人体皮肤模型作为评估药物皮肤渗透性的工具的实用性
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
K. Sugibayashi
中科院分区：
文献类型：
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作者：
Tetsuya Watanabe;T. Hasegawa;Hidekazu Takahashi;T. Ishibashi;K. Takayama;K. Sugibayashi
评价了三维培养的人皮肤模型用于预测药物的皮肤渗透性。不同药物的渗透数据进行了比较，通过离体人和大鼠皮肤。虽然通过培养的人皮肤渗透的每种药物的累积量约为通过切除的人和大鼠皮肤渗透的药物的累积量的10倍，但在这些渗透性中发现了良好的相关性。每种药物的皮肤渗透时间曲线对于两种皮肤膜都非常相似。还评价了用于培养的人皮肤渗透的速率限制层。从这些结果可以清楚地看出，在培养的人皮肤中，总表皮是主要屏障，而在切除的无毛大鼠皮肤中，角质层单独是屏障。
Three-dimensional cultured human skin model was evaluated for prediction of skin permeability of drugs. The permeation data of different drugs were compared with those through excised human and rat skin. Although the cumulative amount of each drug that permeated through the cultured human skin was about 10-fold higher than those through the excised human and rat skin, a good correlation was found in those permeabilities. Time profiles for skin permeation of each drug were very analogical for both the skin membranes. The rate-limiting layer for cultured human skin permeation was also evaluated. It is clear from these results that the total epidermis is the primary barrier in the cultured human skin, whereas the stratum corneum alone is the barrier in the excised hairless rat skin.