Simultaneous determination of dopamine and 3,4-dihydroxyphenylacetic acid in mouse striatum using mixed-mode reversed-phase and cation-exchange high-performance liquid chromatography
Simultaneous determination of dopamine and 3,4-dihydroxyphenylacetic acid in mouse striatum using mixed-mode reversed-phase and cation-exchange high-performance liquid chromatography
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DOI：
10.1016/j.jpba.2009.09.045
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发表时间：
2010-02-05
影响因子：
3.4
通讯作者：
Funatsu, Takashi
中科院分区：
文献类型：
--
作者：
Tsunoda, Makoto;Aoyama, Chiaki;Funatsu, Takashi
多巴胺和3，4-二羟基苯乙酸（DOPAC）的测量是有用的多巴胺周转指数。建立了高效液相色谱-荧光检测法同时测定多巴胺和多巴酚乙酸的方法。采用反相阳离子交换混合柱（CAPCELL PAK CR柱）分离了3，4-二羟基-L-苯丙氨酸、儿茶酚胺（去甲肾上腺素、肾上腺素和多巴胺）和DOPAC。对移动的相的pH和反离子浓度等因素进行了优化。与移动的相的15 mM醋酸钠缓冲液（pH 4.5），分离在22分钟内实现。所开发的方法适用于测定小鼠纹状体中的多巴胺和DOPAC。小鼠纹状体中多巴胺和DOPAC的浓度分别为4.98 +/- 0.66和1.00 +/- 0.11 μ M（n = 10）。(C)2009爱思唯尔有限公司版权所有。
The measurement of dopamine and 3,4-dihydroxyphenylacetic acid (DOPAC) is useful for an index of dopamine turnover. We developed a simultaneous determination method for dopamine and DOPAC with high-performance liquid chromatography-fluorescence detection. Mixed-mode reversed-phase and cation-exchange column (CAPCELL PAK CR column) which contained C18 silica particles and sulfonic acid cation-exchange particles was used for the separation of 3,4-dihydroxy-L-phenylalanine, catecholamines (norepinephrine, epinephrine, and dopamine) and DOPAC. The mobile phase was optimized for factors such as pH and counter ion concentration. With a mobile phase of 15 mM sodium acetate buffer (pH 4.5), separation was achieved within 22 min. The developed method was applicable to the determination of dopamine and DOPAC in mouse striatum. The concentrations of dopamine and DOPAC in mouse striatum were 4.98 +/- 0.66 and 1.00 +/- 0.11 mu M, respectively (n = 10). (C) 2009 Elsevier B.V. All rights reserved.