Isolation and molecular detection of methylotrophic bacteria occurring in the human mouth
Isolation and molecular detection of methylotrophic bacteria occurring in the human mouth
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DOI：
10.1111/j.1462-2920.2005.00805.x
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发表时间：
2005-08-01
影响因子：
5.1
通讯作者：
Wood, AP
中科院分区：
文献类型：
--
作者：
Anesti, V;McDonald, IR;Wood, AP
从志愿者和牙周炎患者的舌头和口腔中分离出不同的甲基营养细菌，并在牙周上和牙周下的牙菌斑中分离到不同的甲基营养细菌。一碳化合物，如口腔中的二甲基硫化物，可能被用作这些生物的生长底物。利用16S rRNA基因序列对芽孢杆菌、干酪短杆菌、磺酸菌、甲基乳杆菌、黄色微球菌和反常变色杆菌的生理特性进行了研究。干酪假单胞菌的模式菌株为甲基营养型。在一些菌株中发现了甲基营养代谢酶的特征，并证明了与已知C-1化合物代谢途径的生长一致的活性。采用聚合酶链式反应技术，从9名志愿者的18份舌样和牙菌斑标本中，分别扩增出甲氧半乳杆菌16S rRNA基因和甲醇脱氢酶mxaF基因。在所有9名志愿者中都检测到了MxaF，在7名志愿者中检测到了甲氧西林杆菌。因此，甲基营养活性是口腔细菌群落的一个特征。
Diverse methylotrophic bacteria were isolated from the tongue, and supra- and subgingival plaque in the mouths of volunteers and patients with periodontitis. One-carbon compounds such as dimethylsulfide in the mouth are likely to be used as growth substrates for these organisms. Methylotrophic strains of Bacillus, Brevibacterium casei, Hyphomicrobium sulfonivorans, Methylobacterium, Micrococcus luteus and Variovorax paradoxus were characterized physiologically and by their 16S rRNA gene sequences. The type strain of B. casei was shown to be methylotrophic. Enzymes of methylotrophic metabolism were characterized in some strains, and activities consistent with growth using known pathways of C-1-compound metabolism demonstrated. Genomic DNA from 18 tongue and dental plaque samples from nine volunteers was amplified by the polymerase chain reaction using primers for the 16S rRNA gene of Methylobacterium and the mxaF gene of methanol dehydrogenase. MxaF was detected in all nine volunteers, and Methylobacterium was detected in seven. Methylotrophic activity is thus a feature of the oral bacterial community.