A novel sandwich-type electrochemical aptasensor for sensitive detection of kanamycin based on GR–PANI and PAMAM–Au nanocomposites
A novel sandwich-type electrochemical aptasensor for sensitive detection of kanamycin based on GR–PANI and PAMAM–Au nanocomposites
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DOI：
10.1039/c4nj00858h
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发表时间：
2014-09
影响因子：
3.3
通讯作者：
Xu Wei;Yu Wang;Su Liu;Jinghua Yu;Hongzhi Wang;Jiadong Huang
中科院分区：
文献类型：
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作者：
Xu Wei;Yu Wang;Su Liu;Jinghua Yu;Hongzhi Wang;Jiadong Huang
在这项工作中，我们报告了一种新型夹心型电化学适体传感器，用于敏感和选择性检测抗生素卡那霉素。该适体传感器是通过在玻璃碳电极表面组装石墨烯-聚苯胺（GR-PANI）和聚酰胺胺树枝状聚合物-金纳米颗粒（PAMAM-Au）纳米复合材料构建的，作为电极材料，其具有优异的电荷传输性能和生物分子负载能力。随后进行夹心式特异性识别，通过HRP催化H2O2电还原反应输出氧化还原电流实现卡那霉素检测。结果表明，所开发的策略具有从 5 × 10−6 至 4 × 10−2 μg mL−1 的宽线性响应范围和 4.6 × 10−6 μg mL−1 的低检测限。此外，该适配体传感器还具有成本低、灵敏度高、特异性好等优点。因此，它可能为卡那霉素测定以及相关食品安全分析和临床诊断提供有用的工具。
In this work, we report a novel sandwich-type electrochemical aptasensor for sensitive and selective detection of antibiotic kanamycin. This aptasensor was constructed by assembling graphene–polyaniline (GR–PANI) and polyamidoamine dendrimer–Au nanoparticle (PAMAM–Au) nanocomposites on the surfaces of a glass carbon electrode, which as electrode materials exhibited excellent charge-transport property and loading capacity of biomolecules. Followed by the sandwich-type specific identification, kanamycin detection was realized by outputting a redox current from electro-reduction reaction of H2O2 catalyzed by HRP. It was demonstrated that the developed strategy had a wide linear response range from 5 × 10−6 to 4 × 10−2 μg mL−1 and a low detection limit of 4.6 × 10−6 μg mL−1. Additionally, this aptasensor had the advantages in its low cost, high sensitivity, and superb specificity. Thus, it might provide a useful tool for kanamycin determination and related food safety analysis and clinical diagnosis.