Characterization of a unique repression system present in arbitrium phages of the SPbeta family
Characterization of a unique repression system present in arbitrium phages of the SPbeta family
复制标题
DOI：
10.1016/j.chom.2023.11.003
复制
发表时间：
2023-12-13
影响因子：
30.3
通讯作者：
Penades, Jose R.
中科院分区：
文献类型：
--
作者：
Brady, Aisling;Cabello-Yeves, Elena;Penades, Jose R.
仲裁蛋白编码肽用于沟通和协调溶解和溶原性之间的决定。然而，这些细菌建立溶原性的机制仍然未知。在这里，专注于SPbeta噬菌体家族的模型SPbeta 3T和SPb，我们报告说，一个六基因操纵子称为“SPbeta阻遏物操纵子”（sro）表达不是一个，而是两个主阻遏物，SroE和SroF，后者折叠像一个经典的噬菌体整合酶。为了促进溶原性，这些阻遏物与噬菌体基因组中的多个位点结合。SroD作为辅助阻遏物，与SroEF一起形成溶原性建立和维持所必需的阻遏模块。此外，操纵子内的蛋白质SroABC被提议构成转换器模块，将任意通信系统连接到阻遏模块的活动。总的来说，这项研究揭示了复杂的和专门的镇压系统所采用的arbitrium SPb样的细胞在裂解溶原性的决定。
Arbitrium-coding phages use peptides to communicate and coordinate the decision between lysis and lysogeny. However, the mechanism by which these phages establish lysogeny remains unknown. Here, focusing on the SPbeta phage family's model phages phi3T and SPb, we report that a six-gene operon called the "SPbeta phages repressor operon"(sro) expresses not one but two master repressors, SroE and SroF, the latter of which folds like a classical phage integrase. To promote lysogeny, these repressors bind to mul-tiple sites in the phage genome. SroD serves as an auxiliary repressor that, with SroEF, forms the repression module necessary for lysogeny establishment and maintenance. Additionally, the proteins SroABC within the operon are proposed to constitute the transducer module, connecting the arbitrium communication system to the activity of the repression module. Overall, this research sheds light on the intricate and specialized repression system employed by arbitrium SPb-like phages in making lysis-lysogeny decisions.