Analyzing cell cycle-dependent degradation and ubiquitination in budding yeast.
Analyzing cell cycle-dependent degradation and ubiquitination in budding yeast.
复制标题
分析芽殖酵母中细胞周期依赖性降解和泛素化。
DOI：
10.1007/978-1-4939-0888-2_17
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Koepp,DeannaM
中科院分区：
文献类型：
--
作者：
Kim,Dong-Hwan;Koepp,DeannaM
细胞周期进程受到严格调控，以防止不受控制的生长和分裂。特定的细胞周期因子负责驱动细胞从一个细胞周期阶段进入下一个阶段。当这些蛋白质的功能不再需要或可能破坏细胞周期进程时，它们中的许多蛋白质被泛素蛋白酶体系统靶向降解。在这里，我们描述了一系列的实验用于研究泛素介导的细胞周期蛋白的降解。这一系列的检测方法可用于确定酿酒酵母细胞周期蛋白降解和泛素化过程中对单个蛋白的需求。
Cell cycle progression is tightly regulated to prevent uncontrolled growth and division. Specific cell cycle factors are responsible for driving the cell from one cell cycle stage to the next. Many of these proteins are targeted for degradation by the ubiquitin proteasome system when their function is no longer required or may disrupt cell cycle progression. Here we describe a series of experiments used to study the ubiquitin-mediated degradation of cell cycle proteins. This collection of assays may be used to determine the requirement for individual proteins in the degradation and ubiquitination of cell cycle proteins inSaccharomyces cerevisiae.