HPLC and HPTLC densitometric determination of andrographolides and antioxidant potential of Andrographis paniculata
HPLC and HPTLC densitometric determination of andrographolides and antioxidant potential of Andrographis paniculata
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DOI：
10.1016/j.jfca.2005.04.007
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发表时间：
2006-03-01
影响因子：
4.3
通讯作者：
Mariam, A
中科院分区：
文献类型：
--
作者：
Akowuah, GA;Zhari, I;Mariam, A
建立了高效液相色谱和反相高效液相色谱联合紫外检测穿心莲中穿心莲内酯和14-脱氧- 11,12 -二脱氢穿心莲内酯两种主要活性物质的快速定量测定方法。HPLC法和HPLC法的加样回收率分别为96.5 ~ 99.0%和98.1 ~ 99.2%。HPLC法的相对标准偏差为0.89 ~ 0.99（日内）和0.86 ~ 0.98（日内），HPLC法的相对标准偏差为0.86 ~ 1.02（日内）和0.87 ~ 1.12（日内）。这些方法用于常规分析，并获得在马来西亚不同地点种植的植物叶片中这两种化合物的相对量。提取物和分离的化合物具有抑制脂质过氧化和自由基活性。(c) 2005爱思唯尔公司版权所有。
A rapid method based on HPTLC and RP-HPLC with UV detection for quantitative determination of two major bioactive compounds in Andrographis paniculata, andrographolide and 14-deoxy-11, 12-didehydroandrographolide, is described. The recoveries of the two compounds were between 96.5-99.0% by HPTLC method and 98.1-99.2% by HPLC assay. The relative standard deviations of the two compounds ranged between 0.89-0.99 (intra-day) and 0.86-0.98 (inter-day) for the HPTLC method and 0.86-1.02 (intra-day) and 0.87-1.12 (inter-day) for HPLC method. The methods were used for routine analyses and to obtain relative amount of the two compounds in the leaves of the plant cultivated in different locations of Malaysia. The extracts and isolated compounds exhibited lipid peroxidation inhibition and free radical activities. (c) 2005 Elsevier Inc. All rights reserved.