The proteinase-activated receptor 2 is involved in nociception
The proteinase-activated receptor 2 is involved in nociception
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DOI：
10.1523/jneurosci.21-22-09036.2001
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发表时间：
2001-11-15
影响因子：
5.3
通讯作者：
Pasricha, PJ
中科院分区：
文献类型：
--
作者：
Hoogerwerf, WA;Zou, L;Pasricha, PJ
蛋白水解酶激活的受体2表达在初级传入神经元的一个亚群上，可能参与炎症的神经源性成分。我们推测，这种受体也可能在神经元敏化中发挥作用，并在炎症性条件下(如胰腺炎)的疼痛发病机制中起作用。使用一种特定的蛋白酶激活受体2激活肽，我们在体外发现了这种敏化的证据，其形式是辣椒素和氯化钾诱导的降钙素基因相关肽的增强释放，降钙素基因相关肽是伤害性信号的标志。通过脊髓背角Fos的表达，我们证明了胰管内注射蛋白酶激活的受体2激活肽可以激活和敏化体内的胰腺特异性传入神经元。这些观察表明，蛋白酶激活的受体2参与了伤害性信号传递，并可能在炎症和疼痛之间提供了一种新的联系。
The proteinase-activated receptor 2 is expressed on a subset of primary afferent neurons and may participate in the neurogenic component of inflammation. We hypothesized that this receptor may also play a role in neuronal sensitization and contribute to the pathogenesis of pain in inflammatory conditions such as pancreatitis. Using a specific proteinase-activated receptor 2 activating peptide, we found evidence of such sensitization in vitro in the form of enhanced capsaicin- and KCl-evoked release of calcitonin gene-related peptide, a marker for nociceptive signaling. We then demonstrated that injection of the proteinase-activated receptor 2 activating peptide into the pancreatic duct can activate and sensitize pancreas-specific afferent neurons in vivo, as measured by Fos expression in the dorsal horn of the spinal cord. These observations suggest that proteinase-activated receptor 2 contributes to nociceptive signaling and may provide a novel link between inflammation and pain.