Directly utilizing an endogenous gene to dissect regulatory elements in the biosynthetic gene cluster of nosiheptide.
Directly utilizing an endogenous gene to dissect regulatory elements in the biosynthetic gene cluster of nosiheptide.
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DOI：
10.1039/c4cc04974h
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发表时间：
2014-08
影响因子：
4.9
通讯作者：
Min Ma;Yanjiu Xue;Weiying Liu;Hong Zhang;L. Kong;Shuzhen Wang;Yijun Chen
中科院分区：
文献类型：
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作者：
Min Ma;Yanjiu Xue;Weiying Liu;Hong Zhang;L. Kong;Shuzhen Wang;Yijun Chen
通过一种新的宿主-载体系统，以生物合成基因簇内的内源基因作为报告基因，鉴定了那西肽生物合成的调控元件。本研究提供了一个快速，可靠的方法来鉴定各种生物活性天然产物的生物合成的调控元件。
The regulatory elements for nosiheptide biosynthesis were identified by a novel host-vector system with an endogenous gene within the biosynthetic gene cluster as a reporter gene. The present study offers a rapid and reliable method for the identification of regulatory elements in the biosynthesis of various bioactive natural products.