RT-PCR determination of maspin and mammaglobin B in peripheral blood of healthy donors and breast cancer patients
RT-PCR determination of maspin and mammaglobin B in peripheral blood of healthy donors and breast cancer patients
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DOI：
10.1093/annonc/mdj109
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发表时间：
2006-03-01
影响因子：
50.5
通讯作者：
Flamini, E
中科院分区：
文献类型：
--
作者：
Mercatali, L;Valenti, V;Flamini, E
背景：本研究的目的是评估两种标志物masmasin和mammaglobin B单独或联合检测乳腺癌的准确性。为了更好地确定这两种标志物在诊断目的上的潜力和局限性，我们分析了血液阳性与临床、病理和肿瘤生物学特征的关系。患者和方法：采用巢式逆转录酶聚合酶链式反应（RT-PCR）检测27名健康供者和140名未接受治疗患者外周血（PB）样本中的标志物。结果：24%的患者血液样本中maspin呈阳性，特异性为89%。对于mammaglobin B，在7%的患者中观察到阳性，而在健康供者中从未观察到阳性。maspin的表达与原发肿瘤细胞增殖相关（P = 0.015），而mammaglobin B的表达与病理分期相关（P = 0.013）。这两种标记物的存在都与淋巴结状态显著相关。结论：我们的研究结果表明，这两种标志物的关联可能代表了一种潜在的有用的非侵入性检测乳腺癌的工具。这些标志物作为高风险复发指标的验证正在一系列患者中进行，并进行了充分的随访。
Background: The aim of the present study was to evaluate the accuracy of two markers, maspin and mammaglobin B, singly or in combination, to detect breast cancer. To define better the potential and limits of the two markers for diagnostic purposes, blood positivity was analyzed in relation to clinical, pathological and biological tumor characteristics.Patients and methods: The markers were determined in peripheral blood (PB) samples from 27 healthy donors and 140 previously untreated patients using nested reverse transcriptase polymerase chain reaction (RT-PCR).Results: Positivity for maspin in blood samples was observed in 24% of patients with an 89% specificity. For mammaglobin B, positivity was observed in 7% of patients and never in healthy donors. The presence of maspin was correlated with cell proliferation of the primary tumor (P = 0.015), whereas mammaglobin B positivity correlated with pathological stage (P = 0.013). The presence of either marker was significantly related to nodal status.Conclusions: Our results indicate that the two markers in association could represent a potentially useful non-invasive tool to detect breast cancer. The validation of these markers as indicators of high risk of relapse is ongoing in a series of patients with an adequate follow-up.