Isolation, identification, and degradation performance of a PFOA-degrading strain.
Isolation, identification, and degradation performance of a PFOA-degrading strain.
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DOI：
10.4238/gmr.15028043
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发表时间：
2016-05
期刊：
影响因子：
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通讯作者：
L. Yi;L. Chai;Y. Xie;Q. Peng;Q. Peng
中科院分区：
文献类型：
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作者：
L. Yi;L. Chai;Y. Xie;Q. Peng;Q. Peng
以全氟辛酸（PFOA）为唯一碳源，通过驯化和富集培养，从某全氟化合物生产厂附近的土壤中分离到一株全氟辛酸（PFOA）降解菌YAB 1。根据菌落形态、生理生化特征和16 S rRNA基因序列分析，初步鉴定该菌株为拟富氏假单胞菌（Pseudomonasparafulva）。摇瓶发酵试验表明，YAB 1对PFOA的最大耐受浓度为1000 mg/L。细菌生长和PFOA降解的最佳条件为30°C，pH 7，2%接种量，PFOA初始浓度为500 mg/L。培养96 h后，GC-MS分析测定的PFOA降解率为32.4%。当无机盐培养基中添加1g/L葡萄糖时，降解率提高到48.1%。葡萄糖是PFOA降解的最佳外源碳源。研究了PFOA降解菌的降解性能。
The perfluorooctanoic acid (PFOA)-degrading strain YAB1 was isolated from the soil near a perfluorinated compound production plant through acclimation and enrichment culture, using PFOA as the sole carbon source. This strain was preliminarily identified as Pseudomonas parafulva based on colony morphology, physiological and biochemical features, and 16S rRNA gene sequencing. Using shaking flask fermentation, the maximum tolerable concentration of YAB1 on PFOA was found to be 1000 mg/L. The optimal conditions for bacterial growth and PFOA degradation were 30°C, pH 7, 2% inoculum, and an initial PFOA concentration of 500 mg/L. After 96 h of culture, the PFOA degradation rate determined by GC-MS analysis was 32.4%. When 1 g/L glucose was added to the inorganic salt culture medium, the degradation rate increased to 48.1%. Glucose was the best exogenous carbon source for the degradation of PFOA. This study reports the degradation performance of PFOA-degrading bacteria.