Reversibly labile, sclerotization-induced elastic properties in a keratin analog from marine snails: whelk egg capsule biopolymer (WECB)
Reversibly labile, sclerotization-induced elastic properties in a keratin analog from marine snails: whelk egg capsule biopolymer (WECB)
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DOI：
10.1242/jeb.02613
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发表时间：
2007-01-01
影响因子：
2.8
通讯作者：
Shadwick, Robert E.
中科院分区：
文献类型：
--
作者：
Rapoport, H. Scott;Shadwick, Robert E.
以两种新腹足类海螺（Busycon canaliculatum和Kelletia kelletii）的卵囊为研究对象，探讨新生卵囊力学性质的成因，并建立该材料的生物力学模型。扫描电子显微镜显示，胶囊在加工过程中的所有阶段都具有纤维状的层次结构，而机械完整性正在发展。这表明腹侧足腺中发生的尚未表征的硬化机制主要将这些纤维成分结合在一起。通过各种物理和化学处理分解WECB的机械行为，使我们开发了这种材料的结构和机械性能的模型，支持其指定为角蛋白类似物。角蛋白力学模型应用于WECB在其表示为无基质的中间丝（IF）型蛋白质和更复杂的复合材料，将IF，如角蛋白之间的中间状态。
Egg capsules from two caenogastropod whelks, Busycon canaliculatum and Kelletia kelletii, were studied to investigate the genesis of mechanical properties of nascent capsules and to formulate a biomechanical model of this material. Scanning electron microscopy revealed that the capsules possess fibrous hierarchical arrangements at all stages during processing while the mechanical integrity is developing. This suggests that an as yet uncharacterized sclerotization mechanism occurring in the ventral pedal gland primarily binds these fibrous components together. Decomposing the mechanical behavior of WECB through various physical and chemical treatments led us to develop a model for the structure and mechanical properties of this material that supports its designation as a keratin analog. Keratin mechanical models were applied to WECB in its representation as an intermediate state between matrix-free intermediate filament (IF)-type proteins and the more complex composite materials incorporating IFs such as keratin.